Sambrook is best known for his studies on DNA tumor viruses and the molecular biology of normal and
neoplastic cells. His Tumour Virus Group at Cold Spring Harbor identified and mapped all of the major genes of
adenoviruses and
SV40, determined their
transcriptional control in infected and transformed cells, and elucidated the mechanism of integration of these viruses into the genome of the host cell. He has also made important contributions to the understanding of
intracellular traffic and
protein folding and is an influential leader in the field of the
molecular genetics of human cancer. Sambrook is a former director of research at the
Peter MacCallum Cancer Centre in Melbourne. He was elected a
Fellow of the Australian Academy of Science in 2000. and is a
Fellow of the Royal Society. He was the founder and director of the Kathleen Cunningham Consortium for research into
familial breast cancer, KConFab, that was established in 1995. Sambrook has published four editions of the best-selling, highly influential laboratory manual
Molecular Cloning, the third in 2001 with David Russell and the fourth in 2012 with Michael R. Green. He is also co-editor of
Inspiring Science: Jim Watson and the Age of DNA and
Life Illuminated: Selected Papers from Cold Spring Harbor Volume 2, 1972–1994. All three books were published by
Cold Spring Harbor Laboratory Press. In 2009 he was awarded the "Victorian Government Leadership and Innovation Award". ==Personal life==