It was established in
Kyiv on 21 October 2015 consisting of
"Kyiv-1" Battalion, "Kyiv-2" Battalion, "Golden Gate" Battalion,
"Sich" Battalion and "St. Mary" Battalion. It conducted law enforcement operations in
Kyiv Oblast especially on 28 and 29 December 2015. On the night of 23–24 March 2016, the Kyiv Regiment saw its first combat against separatists in
Avdiivka. On 14 April 2016, the regiment guarded strategic sites in
Avdiivka. On 22 June 2016, the "St. Mary" battalion was disbanded and its fighters were transferred to other units. On 9 July 2016, it was deployed to Stanytsia Luhansk where they discovered another drug den, 520 opium poppy bushes and 124 hemp bushes were discovered and destroyed. On 11 July 2016, a monument was opened for a volunteer soldier of the Russian-Ukrainian war in
Kyiv by the regiment. In the summer of 2016, the regiment constantly carried out active counter-subversive operations in
Avdiivka. On 31 January 2017, the curator of the unit,
Yevhen Deidei reported that 50 personnel of the regiment were performing operations in
Avdiivka, which had been shelled by separatists for three consecutive days, they protected private property from criminals, evacuated civilians to bomb shelters, and carried out rescue operations as well as collecting aid from the citizens of
Kyiv to help the civilians in Avdiivka. On 25 October 2017, a soldier of the regiment (Kushnir Ruslan Valerievich) was killed as a result of a bomb explosion in Kyiv. During February–March 2022, the regiment took part in the
Northern Ukraine campaign especially the
Battle of Kyiv. On 25 February 2022, a soldier of the
4th "Sich" company (Tsvila Iryna Volodymyrivna) was killed while repelling a tank attack during the
Battle of Irpin. Following the victory in the
Battle of Kyiv, the regiment was deployed to fight in the
Battle of Avdiivka during which on 25 June 2022, a soldier of the regiment (Berezhnyk Ivan Mykolayovych) was killed in combat. On 16 September 2022, it was deployed to fight in the
2022 Kharkiv counteroffensive conducting assault operations in Verbivka and Balakliya. It was then deployed to
Donetsk Oblast and during this deployment a soldier of the regiment (Oleksandr Oleksandrovich Lemak) was killed in combat on 21 October 2022 and another (Grama Ruslan) was killed on 18 March 2023 during the
Battle of Bakhmut. ==Tasks==