Balasubramanian started his research activities in 1965 focusing on the structure and functions of proteins and
polypeptides and worked on the thermodynamic analysis of their stability. His research revealed how
cataract is caused when crystallins are damaged photochemically, thereby leading to diminished lenticular transparency. of the blindness in the world.
Ginko Biloba and
Withania somnifera extracts. These substances contained antioxidants and cytoprotective compounds which slow down the progression of
oxidative cataract and this was verified during experiments in animals. with a sampling set of over 400 families and this has helped in revealing 15 mutations in the
gene CYP1B1, with mutation R368H being the most common one. He and his group have been successful in isolating the adult stem cells found in the
limbus, around the
cornea, and culturing them on human
amniotic membrane. He is credited with over 450 articles, and
Microsoft Academic Search, an online repository of scientific articles, has listed 52 of them. He has presented more than 170 scientific papers On the academic front, he has assisted 16 doctoral students in their PhD studies. His efforts are also reported behind the establishment of a vaccine unit at the
Centre for Cellular and Molecular Biology and in designing a quality improvement program for the
Sericulture Laboratory of the state government. ==Awards and recognitions==