Neutral spray In one version of the laser spray interface, explosive vaporization and
mist formation occur when an
aqueous solution effusing from the tip of the
stainless steel capillary is irradiated from the opposite side of the capillary by a 10.6 μm infrared
laser.
Laserspray ionization Laserspray Ionization (LSI) is a newer mass spectrometric technique commonly used with biomolecules, such as proteins. This method is similar to
matrix-assisted laser desorption/ionization (MALDI) at atmospheric pressure in that it involves an
analyte and
matrix mixture. It also contains features from electrospray ionization, in which it produces a similar mass spectra. The mechanism was initially thought to involve laser induced production of highly charge matrix/analyte clusters that upon evaporation of the matrix produces ions by the same mechanism as ESI. LSI's ability to ablate proteins at atmospheric pressure in order to form a multiple of charged ions with a mass resolution of 100,000 when coupled with a
quadrupole orbitrap mass spectrometer. The advantages of using LSI includes a solvent-free ionization technique, fast data acquisition, simply to use, and the improved fragmentation through multiple charging.
Laser spray inlet ionization Due to recent innovations to the laser spray technique, a new method of laser ablation using the spray method has surfaced. Laserspray inlet ionization (LSII) involves a matrix/analyte sample at atmospheric pressure being
ablated, and the ionization process will take place in an ion transfer capillary tube located in the mass spectrometer
inlet. The LSII method is also known as laserspray ionization vacuum (LSIV). ==Applications==