• William James (1577–1581) • Richard Moodie (1581–?) • Henry Frogge (1583–1587) • John Matthew (1587–1594) (1594–1623) • Thomas Brooke (1623–1629) •
Abraham Wheelocke (1629–1653) • William Moore (1653–1659) • ?James Fenton (1655–?) •
Thomas Smith (1659–1661) • Isaac Dobson (1661–1668) • Robert Peachy (1667–1683) • James Manfield (1684–1686) • John Laughton (1686–1712) • Philip Brooke (1712–1718) • Thomas Macro (1718–1721) • Samuel Hadderton (1721–1731) •
John Taylor (1732–1734) • Thomas Parne (1734–1751) •
Stephen Whisson (1751–1783) •
John Davies (1783–1817) •
Edward Daniel Clarke (1817–1822) •
John Lodge (1822–1845) ==Principal Librarian (Protobibliothecarius), 1721–1828 ==