MALSU1 is a member of the DUF143 family (=
domain of unknown function 143, ) which is highly conserved in both
prokaryotes and
eukaryotes but not
archaea. Examples of mammalian conservation are given below using the ALIGN tool from the
San Diego Supercomputer Center Biology Workbench. Percentages indicate the identity shared by the human protein and the respective mammalian protein. Accession numbers are from the NCBI database. There are no known or predicted paralogs in
Homo sapiens. That is, MALSU1 is a single-copy gene. The domain is from position 93 to 194 on the human protein and comprises 43.2% of the sequence. This conserved domain is also present in the plant gene
iojap, a pattern-striping gene in maize. However, since its function has been solved at least in bacteria, it is no longer a "domain of unknown function".
Protein function While the function of the protein in mitochondria is not conclusive its bacterial homolog has been shown to silence
bacterial translation by blocking the two
ribosomal subunits from joining, hence it was called
RsfS (= ribosomal silencing factor in starvation or stationary phase, a synonym of RsfA).
Protein-protein interactions. RsfS has been shown via a yeast two-hybrid screen to interact with ribosomal protein L14 in four bacterial species as well as in mitochondria. which is part of the
ESCRT-III complex (Endosomal Sorting Complex Required for Transport). DUF143 has also been shown to interact with UFD1, tRNA synthetases class II, and Cytidylyltransferase in various architectures.
Properties Bioinformatics predicted the following properties for LOC_115416: •
Molecular Weight: 26.2 KDal •
Isoelectric Point: 5.155 == Gene ==