Fowler taught music at Prairie View State Normal and Industrial College, and directed a church choir in Fort Worth. She was co-founder of the Texas Association of Negro Musicians in 1926, and she served on the board of the National Association of Negro Musicians, as scholarship committee chair, and edited its journal,
The Negro Musician. In 1928, she started the Mwalimu School. She moved the Mwalimu program to Harlem in 1932, where (as the Mwalimu Center for African Culture) it became a contribution to the
Harlem Renaissance. Literary figures such as
Carter G. Woodson taught at Mwalimu in Harlem. The school also offered a community kitchen, a library of works by black authors, and lessons from bodybuilding to comparative religion. The school's Mwalimu Festival Chorus performed often in New York, and made recordings, under Fowler's direction; they were called "one of the outstanding Negro choral groups in technical proficiency" by
Alain LeRoy Locke. In 1930, a pageant Fowler wrote, produced, and directed,
The Voice, was performed by over 2000 cast members at the National Baptist Convention in Chicago. She also wrote
Up From Slavery, and another musical piece,
African Suite. Several paintings by Manet Harrison Fowler are in the Juneteenth Museum in Fort Worth. A portrait she painted of her daughter Manet Helen Fowler is in the permanent collection at
Yale University Art Gallery. In 1972, Fowler was honored alongside
Duke Ellington,
Ramsey Lewis,
Everett Lee, and
Margaret Rosezarian Harris at the annual awards dinner at the Waldorf Astoria, New York for the National Association of Negro Musicians. ==Personal life==