Main •
Mickey Mouse (voiced by
Wayne Allwine in Seasons 1–3 and
Bret Iwan in Season 4): The optimistic and easygoing leader of The Sensational Six. He is very patient and caring, especially towards his pet dog Pluto. He is Minnie's boyfriend. •
Minnie Mouse (voiced by
Russi Taylor): Mickey Mouse's loving and quick-thinking girlfriend and Daisy's best friend who loves bows. She is often prone to being put in over-the-top experiences. •
Donald Duck (voiced by
Tony Anselmo): Mickey's short-tempered but good-natured best friend, Daisy's boyfriend and co-leader of The Sensational Six. He is often shown to display a short temper that is easily provoked, though the series somewhat downplays this in favor of highlighting his impulsiveness and propensity for unintentional accidents. •
Daisy Duck (voiced by
Tress MacNeille): Donald's girlfriend and Minnie's best friend, who is prone to getting distracted and being talkative. However, she does mean well, and she also knows how to solve many problems and mysteries. •
Goofy (voiced by
Bill Farmer): Mickey's innocent but well-meaning best friend. Being the clumsy guy he is, Goofy is often the source of most of the slapstick presented in this series. •
Pluto (vocal effects provided by Bill Farmer): Mickey's best pet dog who loves adventure. His arch-nemesis is Butch the Bulldog, whose owner is revealed to be Pete. • Toodles (voiced by
Rob Paulsen): A tool-distributing robot that Mickey calls to the Mouseketools for every episode. In Season 3, he gains a face, a personality, and a voice.
Recurring •
Pete (voiced by
Jim Cummings): A friend and foe of Mickey's. In many episodes, he dons an alter-ego and tries to foil whatever plans Mickey and his friends have. He's more mischievous and playful and less malicious. •
Clarabelle Cow (voiced by
April Winchell): A
cow who runs the Moo Mart, a local supermarket. She also runs a nursery show as Mother Goose Clarabelle. •
Ludwig Von Drake (voiced by Corey Burton): A genius inventor who creates many gadgets for Mickey and his friends to use, though some of them backfire. •
Chip (voiced by Tress MacNeille) and
Dale (voiced by Tress MacNeille in "Daisy Bo Peep" and
Corey Burton afterwards): A pair of chipmunks who love digging holes and eating nuts. •
Willie the Giant (voiced by
Will Ryan): A giant who lives on a barn in the clouds. He was portrayed as more antagonistic in Season 1, but was made friendlier for Season 2 onwards. • Martian Mickey (voiced by Wayne Allwine in Seasons 2–3 and Bret Iwan in Season 4): Mickey's alien counterpart from
Mars. • Martian Minnie (voiced by Russi Taylor): Minnie's alien counterpart from Mars. • Pluto From Pluto (voiced by
Bill Farmer): Pluto's counterpart that is originally from
Pluto but is now Martian Mickey's pet. Unlike Pluto, he mainly communicates by making warbling sounds with his tail rather than barking. • Goofbot (voiced by Bill Farmer): A robot counterpart of Goofy that was built by him. •
Santa Claus and
Mrs. Claus (voiced by
Dee Bradley Baker and Tress MacNeille, respectively): Santa is a jolly old man who, along with his wife, Mrs. Claus, visits Mickey and his friends every Christmas Day. They also appeared in the special "Choo Choo Express." • Mrs. Claus (voiced by Tress MacNeille) • Baby Red Bird and Mommy Red Bird (vocal effects both provided by Tress MacNeille): Baby Red Bird was born on a nest that Goofy mistaken for a hat. He usually "tweets" in threes, and was later returned to his mother who has the same tweeting pattern. • Boo Boo Chicken and Coco the Monkey (vocal effects both provided by Dee Bradley Baker): Boo Boo Chicken is Donald's pet chicken. Coco the Monkey is Goofy's monkey friend from the jungle. •
Figaro,
Butch the Bulldog, Mr. Pettibone, and Bella (vocal effects all provided by
Frank Welker): Figaro and Mr. Pettibone are the respective cats of Minnie and Goofy, while Butch and Bella are the respective dogs of Pete and Clarabelle. • Quoodles (voiced by Russi Taylor): Toodles' female counterpart and love interest. She originally worked for Space Pirate Pete but now acts as Minnie's assistant and gives her Quoodlestools whenever Toodles is busy with Mickey.
Guests •
Mortimer Mouse (voiced by
Maurice LaMarche): A mouse who was originally a supervillain known as Mega Mort. • Captain Goof-Beard (voiced by
Dick Van Dyke): A pirate and Goofy's grandfather who lives on Harmony Chord Island. •
Millie and
Melody Mouse (voiced by
Avalon Robbins and
Grace Kaufman, respectively): Minnie Mouse's nieces. • The Singing Lock (voiced by
Nika Futterman): A lock that appears on the front door as a result of the Silly Switch in "Mickey's Silly Problem". • Count Mickula (voiced by Bret Iwan): A vampire counterpart of Mickey. He can fly and turn into a bat. • Boodles (voiced by
Chloë Grace Moretz): Toodles' counterpart from Count Mikula's castle who gives Monster Mousketools. She likes to scare people when she is called. • Igor the Door (voiced by
David Tennant): A talking door knocker who operates the front door to Count Mikula's castle. • Cuckoo the Cuckoo Bird (vocal effects provided by Dee Bradley Baker): A
cuckoo bird that escapes his clock, prompting Mickey and Donald to chase after him. • Goofles (voiced by Bill Farmer): Toodles' cousin who resembles and behaves like Goofy. Toodles is able to transfer his Mouseketools to him. ==Production==