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Middlebrook 7H10 Agar

Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Composition
Ingredients (g/L) • Ammonium sulfate, 0.50 • Monopotassium phosphate, 1.50 • Disodium phosphate, 1.50 • Sodium citrate, 0.40 • Magnesium sulfate, 0.025 • Zinc sulfate, 0.001 • Copper sulfate, 0.001 • L-Glutamic acid, 0.50 • Ferric ammonium citrate, 0.04 • Pyridoxine hydrochloride, 0.001 • Biotin, 0.0005 • Malachite green, 0.00025 • Agar, 15.00 • Calcium chloride, 0.0005 Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks. ==See also==
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