Main • Miss Flora Spider (voiced by
Kristin Davis in Seasons 1 and 2, Caroly Larson in Season 3 in the Canadian version and
Maria Darling in the UK version) is an adoptee, adopted by Betty Beetle when her biological mother abandoned her before she hatched and Holley's wife. Miss Spider tries to be a kind and caring mother and gives equal time to all of her eight children. She strongly believes that "We have to be good to bugs, all bugs." • Squirt (voiced by
Scott Beaudin in the Canadian version and
Joanna Ruiz in the UK version) is a curious and very adventurous lime green spider, with a party hat on top. Squirt is considered to be the leader of the children and as such, more stories that focus on him than any other. He really enjoys surfing the air on his webs, dreams of flying like Shimmer or Dragon, and is often ready to take the lead in an adventure. Because of his impulsive and curious personality, he often needs the advice of his parents to help him with certain situations. • Shimmer (voiced by Rebecca Brenner in the Canadian version and Maria Darling in the UK version) is a pink
jewel beetle with an interest in sports and games. Shimmer is quite different from the rest of the family as she has abilities the others do not such as heat sensors. She is one of Miss Spider and Holley's adoptive children. • Dragon (voiced by Mitchell Eisner in the Canadian version and Maria Darling in the UK version) is a purple
dragonfly who is one of Miss Spider and Holley's adoptive children. He is the oldest of the Spider children and often very brash towards the others, especially Squirt. In many episodes, Dragon serves as an example of peer pressure. For example, in "Cry Buggie", Dragon makes fun of Squirt when he shows his feelings. However, he still loves his family. • Bounce (voiced by
Julie Lemieux in the Canadian version and Joanna Ruiz in the UK version) is a blue
bedbug who is one of Miss Spider and Holley's adoptive children. He is a two-legged, high-energy bug and the only bug character in the show to have teeth. He and Dragon have been "best bug buddies" ever since they met because of their similar experiences regarding their native families.
Supporting • Holley (voiced by
Robert Smith in the Canadian version and
David Holt in the UK version) is Miss Spider's best husband. Holley is a great musician who loves to play the guitar. He has a special thinking stone where he makes decisions. • Spinner (voiced by Austin Di Iulio in Seasons 1 and 2,
Cameron Ansell in Season 3 in the Canadian version and Lynn Cleckner in the UK version) is a yellow spider with very large eyeglasses just like Holley's. He looks almost exactly like him, with Miss Spider's coloring. He is a very smart and wise bug often looked to for advice. While he isn't great at physical activities, he has a wonderful talent for the "bugpipes". • Pansy (voiced by
Aaryn Doyle in the Canadian version and Maria Darling in the UK version) and Snowdrop (voiced by Alexandra Lai in the Canadian version and Lynn Cleckner in the UK version) are orange spiders who are two identical twins. Pansy sings well, but not as naturally as Snowdrop sings yet. Pansy wears a purple bow while Snowdrop's is green. • Wiggle (voiced by Marc McMulkin in the Canadian version and Lynn Cleckner in the UK version) is a light blue spider who tends to worry the most out of the group, and is quite emotional. • Ned (voiced by
Jonathan Wilson) and Ted (voiced by Philip Williams) are Australian-accented and
New York-accented fire ant brothers. Ned has a tuft of hair between his antenna. Both of them are scheming, mischievous and deceptive, and have only two main interests: food and getting more of it. A common running gag is that when Ted is mistaken for Ned or vice versa, they would say "I'm Ted. He's Ned." or vice versa. • Stinky (voiced by
Scott McCord in the Canadian version and
David Holt in the UK version) is a green New York-accented
stinkbug who takes up detective work in his free time. He is also very talented at playing the
xylophone. • Felix (voiced by Richard Binsley) is a young and giant
leopard frog who longed to be a bug himself and refuses to eat bugs, as he never finds them yummy. Instead, he eats only berries.
Recurring • Betty Beetle (voiced by
Patricia Gage and
Barbara Budd in the Canadian version and Maria Darling in the UK version) is Miss Spider's adoptive mother, the spiderlings' maternal grandmother, and Holley's mother-in-law. It is revealed that when Miss Spider couldn't find her actual mother, Betty decided to adopt her. • Uncle Asparagus "Gus" (voiced by
Peter Oldring in the Canadian version and David Holt in the UK version) is an orange and green jewel beetle who is Miss Spider's adoptive brother, Betty's son, Holley's brother-in-law, and the spiderlings' maternal uncle. He really likes to stargaze with his nephews and nieces. • Shelley (voiced by
Cole Caplan) is a snail who is good friends with the Spider family. He is also part of the Sunny Patch Bug Scouts. • Spindella (voiced by Kristina Nicoll in the Canadian version and Lynn Cleckner in the UK version) is Spiderus' wife who he met in the Snakey Woods near the farm. • Mandrake (voiced by Samson Weiss-Willis) is Spiderus and Spindella's son. • Bella (voiced by Emma Pustil) and Ivy (voiced by
Nissae Isen) are Spiderus and Spindella's twin daughters. • Grub (voiced by
Robert Tinkler) is a
larva that has told Squirt about the Snakey Woods. • Whiffy (voiced by Judy Marshak) is a light green stinkbug who is Stinky's sister. • Grace (voiced by
Hannah Endicott-Douglas) is an orphaned baby ladybug who was adopted by Stinky and Whiffy. • Lily (voiced by
Stephanie Morgenstern) is an adult ladybug. • Snack (voiced by
Amanda Soha) is a juvenile ladybug. • Eunice Earwig (voiced by
Cara Pifko) is one of the Spider family's neighbors and the mother of Eddy and Lil Sis. • Eddy Earwig (voiced by Ezra Perlman in the Canadian version and Maria Darling in the UK version) is Eunice Earwig's son and oldest child. He is good friends with Squirt. • Lil Sis (voiced by
Tajja Isen) is Eunice's youngest child and Eddie's younger sister. • Mr. Mantis (voiced by
Wayne Robson in the Canadian version and
Tom Eastwood in the UK version) is the teacher of the spiderlings' class. • Beatrice (voiced by Catherine Gallant) is a
queen bee in a hive part of the Hollow Tree. • Honey (voiced by Isabel de Carteret) is the princess of the Hollow Tree and the daughter of Beatrice. She loves honey and hanging out with her friends. • Cookie (voiced by
Susan Roman) is an orphaned
monarch butterfly.
Villains • Spiderus (voiced by
Tony Jay in the Canadian version and Andy Turvey in the UK version) is a short-tempered, stubborn, conniving, arrogant, and cowardly white spider and Spindella's husband. Despite this, he cares for his family and others. He lives in a crack at the bottom of the Hollow Tree. • Mama Snake (voiced by
Alison Sealy-Smith) is a
corn snake that terrorizes the inhabitants of Sunny Patch. • Baby Snake is the son of Mama Snake. ==Production, broadcast and release==