The southernment segment of MS 407 begins at MS 12 west of McCool. The highway starts at a
Y-intersection but soon curves to the east. The road passes a few homes before intersecting
MS 411 and forming a
concurrency towards town. The highway crosses
Yockanookany River and enters the town limits of McCool. The two highways pass the
town hall, a post office, a church, and houses before reaching the center of town where MS 411 breaks off to the south along Allen Street. MS 407 continues east, then curves to the northeast. After passing County Road 2026 (CR 2026) still within the town limits, state maintenance of MS 407 ends, MS 407's northern segment begins in the town of French Camp at MS 413, about west of the latter's intersection with the
Natchez Trace Parkway. MS 407 heads west northwest (but signed as north) out of the town limits past a few homes and between a pair of high voltage
electrical substations. After heading past woods, open fields, and houses, the road enters
Montgomery County. The highway passes through a rural area, intersecting the unsigned MS 43 at its northern terminus in the county. It reaches the settlement of
Poplar Creek where some houses are located along the highway. After curving more to the northwest, MS 407 crosses the
Big Black River before making a sweeping curve towards the west. The road briefly enters the northeasternmost portion of
Carroll County where it heads through an unnamed settlement centered about its intersection with CR 430. Curving to the north, MS 407 reenters Montgomery County heading past open fields before turning to the northwest and heading through forest land. The state highway enters the city of Winona, but in its far reaches where sparse housing and some open fields are found along the road. The road heads through a clearing where it crosses a small creek and heads past the edge of
Winona-Montgomery County Airport. After crossing a railroad at-grade, MS 407 heads through a more defined residential neighborhood. Before ending, MS 407 gains a
center turn lane and passes an elementary school before reaching a
T-intersection with US 51. ==History==