The source, Bucket Corner (sometimes Bucket's Corner), is located to the west of
Chandler's Ford, north of St John the Baptist Church,
North Baddesley (at ). The stream flows initially north-eastwards, past
Trodds Copse Site of Special Scientific Interest, turning east when it meets the
Eastleigh to Romsey railway line to run parallel to the railway. Both river and railway turn southeastwards as they enter Chandler's Ford itself, with Monks Brook crossing under the railway to run along the north bank of a cutting before they pass underneath Flexford Road. Here, a tributary joins the main stream from the north and the brook turns to the south, once again crossing under the railway. The brook continues to run roughly parallel to the railway line until shortly before
Chandler's Ford railway station, where another tributary joins from the west and the brook flows underneath the railway line and the Hursley Road/Winchester Road
roundabout in Chandler's Ford. The brook then continues to flow southeastwards on the north side of the railway line before turning south away from the railway as they approach the
M3 motorway. Monks Brook passes under Weardale Road and flows southwards between Skipton Road and Steele Close before entering a short tunnel that takes it under Leigh Road (the
A335), then subsequent tunnels under the M3 motorway and junction 13
sliproad and into Fleming Park in
Eastleigh. Here the brook continues to flow mainly due south, between Magpie Lane and Passfield Avenue, crossing under Nightingale Avenue on the way. After passing under Chestnut Avenue, Monks Brook continues to flow southwards with Monks Brook Close immediately to the east and Stoneham Lane on the west. There is a flow measuring station on Monks Brook at Stoneham Lane, managed by the
Environment Agency on behalf of the
Centre for Ecology and Hydrology's National River Flow Archive. At this point, the brook has an average flow of per second. The brook continues to flow south, parallel to Stoneham Lane, and passing close to the lakes of
Lakeside Country Park. The brook then passes under the A335 close to
Southampton Airport and under the
M27 motorway just to the east of Junction 5. (In fact, the brook passes under the sliproads of that junction too). The brook continues to flow south before turning slightly southwestwards to run parallel with another railway, the
South West main line, which the brook eventually flows under and emerges alongside the Fleming Arms public house (named after the arms of
Thomas Fleming) in
Swaythling, on the
Mansbridge (
A27) road. After flowing under the A27, the brook continues southwards, passing the rear of
South Stoneham church, entering the grounds of the
Wessex Lane Halls. Here, Monks Brook was a feature of the gardens of
South Stoneham House, landscaped by
Capability Brown and feeds a large salmon pool. It is from these grounds that the brook flows into the
River Itchen, at
Woodmill opposite the upper section of
Southampton's Riverside Park (at ). ==Natural history==