Multifocal multiphoton microscopy is a microscopy technique for generating 3D images, which uses a laser beam, separated by an array of microlenses into a number of beamlets, focused on the sample. The multiple signals are imaged onto a CCD camera in the same way as in a conventional microscope. The image rate is determined by the camera frame rate, depending on the readout rate and the number of pixels and may range well above 30 images/s.