National Collection of Type Cultures (NCTC) is one of the four culture collections that constitutes the Culture Collections operated by the UK Health Security Agency. It is a non-profit culture collection repository located in the UK. NCTC maintains over 5100 bacterial cultures, over 100 Mycoplasmas and more than 500 plasmids, host strains, bacteriophages, and transposons. Of these, 150 strains are available from NCTC in the form of bacterial DNA, supplied at 2 μg, making them suitable for whole genome sequencing amongst other molecular applications. The cultures represent bacteria from widespread geographical locations and are transported in fused-glass ampoules.