As a young man, Nicolas-Théodore accompanied his father on his
Alpine expeditions, some of them under arduous conditions, and assisted him with experiments in physics, chemistry, mineralogy, and meteorology. In one experiment, Nicolas-Théodore confirmed
Boyle's law by a new method: He carefully weighed a tightly closed flask at many different altitudes and found that the differences in weight were exactly proportional to the differences in barometric pressure readings. In other research in the physical sciences, he named the mineral
dolomite after
Déodat Gratet de Dolomieu, in March 1792. Nicolas-Théodore was attracted to chemistry by Lavoisier's discoveries, and he adopted Lavoisier's new system of chemistry early in life. He became interested in the chemistry and physiology of plants, including gas exchange and the ways that different soils affected their growth. His early papers on these subjects laid the groundwork for some of the chapters in his magnum opus,
Recherches chimiques sur la Végétation ("Chemical Research on Plant Growth"), published in 1804. In addition, Saussure demonstrated that plants obtain their carbon from the carbon dioxide in the atmosphere, not through uptake from
humus in the soil, as his immediate predecessors in photosynthesis research had generally believed. Based on his accomplishments in plant chemistry and physiology, Saussure is considered the last of the major early pioneers of photosynthesis research, completing the work begun by his predecessors, including
Jan Baptist van Helmont,
Joseph Priestley,
Jan Ingenhousz, and
Jean Senebier. For the several decades following publication of Saussure's book, his findings about the atmospheric source of plant carbon and the soil source for plant mineral nutrients were largely neglected, and little progress was made in further unraveling the chemical processes within plants. Then, Saussure's findings were re-discovered and revived by the eminent German chemist
Justus von Liebig. In addition, field research by French agricultural chemist
Jean-Baptiste Boussingault substantiated Saussure's conclusions on the importance of mineral nutrients that plants take up from the soil. Saussure's findings have had a significant impact on many disciplines, including chemistry, agriculture, agronomy, soil science, plant physiology, and plant nutrition. He is considered one of the pioneers of modern agriculture. In addition to his studies in plant physiology, Nicolas-Théodore made important advances in the analysis of organic substances. He determined the composition of
alcohol and
ether, and studied
fermentation, the conversion of
starches into
sugars, and many other biochemical processes. In 1815 he was one of the founding members of
Société Helvétique des Sciences Naturelles (
Swiss Academy of Natural Sciences). ==Honours==