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Nonsynaptic plasticity

Nonsynaptic plasticity is a form of neuroplasticity that involves modification of ion channel function in the axon, dendrites, and cell body that results in specific changes in the integration of excitatory postsynaptic potentials and inhibitory postsynaptic potentials. Nonsynaptic plasticity is a modification of the intrinsic excitability of the neuron. It interacts with synaptic plasticity, but it is considered a separate entity from synaptic plasticity. Intrinsic modification of the electrical properties of neurons plays a role in many aspects of plasticity from homeostatic plasticity to learning and memory itself. Nonsynaptic plasticity affects synaptic integration, subthreshold propagation, spike generation, and other fundamental mechanisms of neurons at the cellular level. These individual neuronal alterations can result in changes in higher brain function, especially learning and memory. However, as an emerging field in neuroscience, much of the knowledge about nonsynaptic plasticity is uncertain and still requires further investigation to better define its role in brain function and behavior.

Vs. synaptic plasticity
Neuroplasticity is the ability of a particular part or region of a neuron to change in strength over time. There are two largely recognized categories of plasticity: synaptic and nonsynaptic. Synaptic plasticity deals directly with the strength of the connection between two neurons, including amount of neurotransmitter released from the presynaptic neuron, and the response generated in the postsynaptic neuron. Nonsynaptic plasticity involves modification of neuronal excitability in the axon, dendrites, and soma of an individual neuron, remote from the synapse. Synaptic plasticity Synaptic plasticity is the ability of a synapse between two neurons to change in strength over time. Synaptic plasticity is caused by changes in use of the synaptic pathway, namely, the frequency of synaptic potentials and the receptors used to relay chemical signals. Synaptic plasticity plays a large role in learning and memory in the brain. Synaptic plasticity can occur through intrinsic mechanisms, in which changes in synapse strength occur because of its own activity, or through extrinsic mechanisms, in which the changes in synapse strength occur via other neural pathways. Short-term inhibitory synaptic plasticity often occurs because of limited neurotransmitter supply at the synapse, and long-term inhibition can occur through decreased receptor expression in the postsynaptic cell. Short-term complementary synaptic plasticity often occurs because of residual or increased ion flow in either the presynaptic or postsynaptic terminal, while long-term synaptic plasticity can occur through the increased production of AMPA and NMDA glutamate receptors, among others, in the postsynaptic cell. Nonsynaptic plasticity In comparison, nonsynaptic plasticity is a less well known and somewhat new and ongoing field of research in neuroscience. It is manifested through changes in the characteristics of nonsynaptic structures such as the soma (biology), the axon, or the dendrites. Nonsynaptic plasticity can have short-term or long-term effects. One way these changes occur is through modification of voltage-gated channels in the dendrites and axon, which changes the interpretation of excitatory or inhibitory potentials propagated to the cell. For example, axonal nonsynaptic plasticity can be observed when an action potential fails to reach the presynaptic terminal due to low conduction or buildup of ions. Nonsynaptic dendritic plasticity also adds to the effects of synaptic plasticity through widening of the action potential. As will be discussed further, brain-derived neurotrophic factor (BNDF) is produced by neurons to coordinate nonsynaptic and synaptic plasticity. Nonsynaptic changes in the somal body, axon, or dendrites of the neuron are inextricably linked to synaptic strength. Integration in memory and learning Although much more is known about the role of synaptic plasticity in memory and learning, both synaptic and nonsynaptic plasticity are essential to memory and learning in the brain. There is much evidence that the two mechanisms both work to achieve the observed effects synergistically. A key example of this is memory formation in the synapse, in which modification of presynaptic release mechanisms and postsynaptic receptors affects either long-term potentiation or depression. Continuous somal depolarization, on the other hand, has been proposed as a method for learned behavior and memory by nonsynaptic plasticity. Nonsynaptic plasticity also augments the effectiveness of synaptic memory formation by regulation of voltage-gated ion channels. Nonsynaptic plasticity is the mechanism responsible for modifications of these channels in the axon, leading to a change in strength of the neuronal action potential, invariably affecting the strength of synaptic mechanisms, and thus the depth and length of memory encoding. Regulation of synaptic plasticity Nonsynaptic plasticity also has the ability to regulate the effects of synaptic plasticity through negative feedback mechanisms. Change in the number and properties of ion channels in the axon or dendrites has the ability to diminish the effects of a hyperstimulated synapse. Intrinsic mechanisms Nonsynaptic neuronal areas such as the axon also have inherent qualities that affect the synapse. These essential mechanisms include the delay in depolarization that action potential undergoes while traveling down the axon. This intrinsic quality slows the propagation of action potentials and is due to the movement of depolarizing current down the cytoplasm and the intermittent placement of sodium channels on the Nodes of Ranvier. These mechanisms always exist, but may change depending on the conditions of the cell soma, axon, and dendrites at the time. Therefore, latency, or delay in propagation of action potentials or excitatory postsynaptic potentials, can be variable. Every excitatory postsynaptic potential that is propagated to a postsynaptic cell is first transmitted through the action potential down the axon in the presynaptic cell, and thus nonsynaptic plasticity inherently affects synaptic plasticity. ==Types==
Types
Intrinsic excitability of a neuron The excitability of a neuron at any point depends on the internal and external conditions of the cell at the time of stimulation. Since a neuron typically receives multiple incoming signals at a time, the propagation of an action potential depends on the integration of all the incoming excitatory and inhibitory postsynaptic potentials arriving at the axon hillock. If the summation of all excitatory and inhibitory signals depolarize the cell membrane to the threshold voltage, an action potential is fired. Changing the intrinsic excitability of a neuron will change that neuron's function. Spike generation Nonsynaptic plasticity has an excitatory effect on the generation of spikes. The increase in spike generation has been correlated with a decrease in the spike threshold, Regulation of synaptic plasticity Nonsynaptic plasticity has been linked with synaptic plasticity, via both synergistic and regulatory mechanisms. The degree of synaptic modification determines the polarity of nonsynaptic changes, affecting the change in cellular excitability. Moderate levels of synaptic plasticity produce nonsynaptic changes that will synergistically act with the synaptic mechanisms to strengthen a response. Conversely, more robust levels of synaptic plasticity will produce nonsynaptic responses that will act as a negative feedback mechanism. The negative feedback mechanisms work to protect against saturation or suppression of the circuit activity as a whole. Shunting Shunting is a process in which axonal ion channels open during the passive flow (not requiring an ion pump) of a subthreshold depolarization down the axon. Usually occurring at axonal branch points, the timing of these channels opening as the subthreshold signal arrives in the area causes a hyperpolarization to be introduced to the passively flowing depolarization. Therefore, the cell is able to control which branches of the axon the subthreshold depolarization current flows through, resulting in some branches of the axon being more hyperpolarized than others. These differing membrane potentials cause certain areas of the neuron to be more excitable than others, based on the specific location and occurrence of shunting. High frequency stimulation Short-term effects: High frequency stimulation of a neuron for a short period of time increases the excitability of the neuron by lowering the amount of voltage required to fire an action potential. This results in a major decrease in axonal sodium channels, which are necessary for action potential propagation. If the stimulation continues, eventually the neuron will stop transmitting action potentials and will die. Neuronal death due to overstimulation is called excitotoxicity. Low frequency stimulation Short-term effects: All living neurons have a basal rate of action potential propagation and synaptic release. Thus, low frequency stimulation of a neuron in the short term is similar to the activity of a neuron at rest in the brain. No major changes happen to the intrinsic excitability of the neuron. Long-term effects: Low frequency stimulation of a neuron for a long period of time decreases the excitability of the neuron by activating calcium-dependent phosphatases that tag AMPA receptors for internalization. Low frequency stimulation leads to low levels of calcium in the cell. When calcium concentrations are low, active calcium-dependent phosphatases dominate over calcium-dependent kinases. As more phosphatases are activated, they tag more AMPA receptors for internalization through endocytosis. Since AMPA receptors are one of the main excitatory receptors on neurons, removing them from the cell membrane effectively depresses the cell (if the cell cannot react to excitatory signals, it cannot generate an action potential of its own). In this way low frequency stimulation can actually reverse the effects of long-term potentiation, however these concepts are generally considered types of synaptic plasticity. Homeostatic and Hebbian plasticity Central nervous system (CNS) neurons integrate signals from many neurons. In the short term, it is important to have changes in activity of the neuron because this is how information is conveyed in the nervous system (Hebbian plasticity). However, for long-term sustainability, drift towards excitability or inexcitability will disturb the circuit's ability to convey information (homeostatic plasticity). Long-term potentiation (LTP) induces a higher firing rate in post synaptic neurons. It has been hypothesized that the intrinsic properties of a neuron should be arranged to make the most of the dynamic range, acting as a homeostatic mechanism. However, it was shown that intrinsic excitability follows a lognormal distribution which requires active, Hebbian learning to be kept up. In vitro studies have found that when the spontaneous activity of neuronal cultures is inhibited, the neurons become hyper excitable and that when an increase in activity is induced for long periods, the firing rates of the culture drop. In contrast, there is a wealth of evidence that the opposite form of regulation, Hebbian learning or LTP-IE/LTD-IE, also occurs and theoretical arguments show that Hebbian plasticity must be the dominant form of plasticity for intrinsic excitability as well. an earlier view suggesting that homeostatic plasticity and intrinsic plasticity are linked was shown to be inconsistent with evidence. Mechanism One mechanism for preserving the dynamic range of a neuron is synaptic scaling, a homeostatic form of plasticity that restores neuronal activity to its normal 'baseline' levels by changing the postsynaptic response of synapses of a neuron as a function of activity. Homeostatic modulation of the intrinsic excitability of a neuron is another way to maintain stability. The regulation of ionic conductances can be achieved in a number of ways, mostly through the release of neuromodulators like dopamine, serotonin etc. Another way is through the controlled release of brain-derived neurotrophic factor (BDNF). BDNF has also been found to influence synaptic scaling, suggesting that this neurotrophic factor may be responsible for the coordination of synaptic and nonsynaptic mechanisms in homeostatic plasticity. Dendritic excitability is important for the propagation and integration of synaptic signals. Dendritic excitability is thought to contribute to E-S potentiation, or an increase in the probability that a given input will result in the firing of an action potential. It is known that changes in dendritic excitability affect action potential back propagation. Action potentials begin near the axon hillock and propagate down the length of the axon, but they also propagate backward through the soma into the dendritic arbor. Active back propagation is dependent on ion channels and changing the densities or properties of these channels can influence the degree to which the signal is attenuated. Back propagation is a method of signaling to the synapses that an action potential was fired. This is important for spike-timing-dependent plasticity. Fast dendritic adaptation on timescales of few seconds was experimentally observed indicating a potential meaningful global learning mechanism Intrinsic plasticity Intrinsic plasticity is a form of activity-dependent plasticity distinct from synaptic plasticity, which involves changes at the synapse between two neurons rather than changes in the electrical properties within a single neuron. There are some closely related phenomena that can affect a neuron's excitability – such as neuromodulation, structural plasticity, short-term plasticity due to channel kinetics, and neural development. There is no consensus on the quantity that intrinsic plasticity regulates, e.g. the firing rate of a neuron, its gain or its internal calcium concentration. Functionally, intrinsic plasticity might allow neurons to learn the intensity of stimuli and represent those intensity statistics in their excitabilities. Intrinsic plasticity contributes to encoding memory and complements other forms of activity-dependent plasticity including synaptic plasticity. ==Higher brain function==
Higher brain function
Long-term associative memory Experimental evidence The experiment of Kemenes et al. demonstrated that in an extrinsic modulatory neuron, nonsynaptic plasticity influences the expression of long-term associative memory. The relationship between nonsynaptic plasticity and memory was assessed using cerebral giant cells (CGCs). Depolarization from conditioned stimuli increased the neuronal network response. This depolarization lasted as long as the long-term memory. Persistent depolarization and behavioral memory expression occurred more than 24 hours after training, indicating long-term effects. In this experiment, the electrophysiological expression of the long-term memory trace was a conditioned stimulus induced feeding response. CGCs were significantly more depolarized in the trained organisms than the control group, indicating association with learning and excitability changes. When CGCs were depolarized, they showed an increased response to the conditional stimuli and a stronger fictive feeding response. This demonstrated that the depolarization is enough to produce a significant feeding response to the conditioned stimuli. Additionally, no significant difference was observed in the feeding rates between conditioned organisms and ones that were artificially depolarized, reaffirming that depolarization is sufficient to generate the behavior associated with long-term memory. demonstrated that eyeblink conditioning (EBC), a form of classical conditioning for studying neural structures and mechanisms underlying learning and memory, in a cat is associated with increased excitability and input in the neurons in sensorimotor cortical areas and in the facial nucleus. It was observed that increasing excitability from classical conditioning continued after the response stopped. This suggests that increased excitability may function as a mechanism for memory storage. Substance dependence Drugs of abuse typically affect the mesolimbic system, or more specifically, the reward pathway of the nervous system. Amongst the common drugs of abuse, nicotine is one of the strongest agonists at the nicotinic cholinergic synapse. Nicotine, competing with acetylcholine (ACh), acts through the nonsynaptic, preterminal, nicotinic acetylcholine receptor (nAChRs) to initiate a membrane potential change and propagate an intracellular Ca2+ signal, thus encouraging the release of neurotransmitters. The specific and characteristic role of calcium current mediated nAChR activity has a different voltage-dependence than other Ca2+ permeable ion channels, as well as different temporal and spatial distribution and as a result, the nonsynaptic nAChR activity enhances the induction of synaptic potentiation, promoting the learning of substance dependence. ==Applications to disease==
Applications to disease
After damage Nonsynaptic plasticity can function to alleviate the effects of brain damage. When one of the vestibular nerves is damaged, disparity in the firing rates of neurons in the vestibular nuclei causes unnecessary vestibular reflexes. The symptoms of this damage fade over time. This is likely due to modifications of intrinsic excitability in the neurons of the vestibular nucleus. Seizure activity Nonsynaptic plasticity also plays a key role in seizure activity. Febrile seizures, seizures due to fever early in life, can lead to increased excitability of hippocampal neurons. These neurons become highly sensitized to convulsant agents. It has been shown that seizures early in life can predispose one to more seizures through nonsynaptic mechanisms. Trauma, including stroke that results in cortical injury, often results in epilepsy. Increased excitability and NMDA conductances result in epileptic activity, suggesting that nonsynaptic plasticity may be the mechanism through which epilepsy is induced after trauma. Autism Valproic acid (VPA) is a treatment for epilepsy, migraines, and bipolar disorder that has been linked to many conditions including autism. An animal model of autism exists in which pregnant rats are given VPA. The offspring have traits similar to those of humans with autism. Shortly after birth, these animals exhibit decreased excitability and increased NMDA currents. These effects are corrected at later stages in life. The changes in intrinsic excitability in these animals helped to offset the effects of increased NMDA currents on network activity, a form of homeostatic plasticity. It is believed that this helps mediate the detrimental effects that the increased NMDA currents would have. ==Current and future research==
Current and future research
Additional research is needed to obtain a broader understanding of nonsynaptic plasticity. Topics that should be further explored include: • Local versus global excitability changes in neuronal networks and maintenance of the memory trace == References ==
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