On 31 October 2016, researcher Leanda Mason discovered Number 16's burrow in disrepair. The spider was gone. Evidence suggested she was killed by a parasitic
spider wasp which had pierced the silk plug of her burrow. During a survey six months earlier, Number 16 had been alive. Mason stated that "She was cut down in her prime[...] It took a while to sink in". The spider's death received widespread publicity in late April 2018, with the publication of a research article in the journal
Pacific Conservation Biology. Based on the burrow fidelity of females of her species, the researchers concluded with a "high level of certainty" that Number 16 was 43 years old at the time of her death. After retiring,
Barbara York Main moved to a care facility for
Alzheimer's disease. Leanda Mason, who kept in contact with her mentor, said in 2018 that Barbara "remembers No. 16" but "forgets that she's died." ==References==