Highway 101 begins in the west at Highway 17 near the town of Wawa and the village of
Michipicoten, within
Algoma District. Initially proceeding north, the route quickly turns eastward in Wawa and enters the barren expanses of the
boreal forest, travelling through uninhabited wilderness for much of its journey across the
Canadian Shield. Approximately east of Wawa, the route encounters
Highway 547, which travels north to
Hawk Junction. From that junction, Highway 101 travels south for several kilometres before resuming its east–west orientation. Just east of
The Shoals Provincial Park, the highway crosses the
Laurentian Divide, the boundary between the Great Lakes and Arctic Ocean
watersheds; a sign and a small picnic area mark the transition. Approximately midway between Wawa and Chapleau, the route enters
Sudbury District, through which it continues most of the distance to Timmins. Approaching Chapleau, Highway 101 encounters Highway 129, onto which it turns northward and becomes
concurrent for . Prior to entering the town, Highway 101 branches eastward, passing north of
Chapleau Airport and resuming its course through the wilderness. Midway between Chapleau and Timmins, the route passes the village of
Foleyet. It briefly travels through
Timiskaming District before entering
Cochrane District, through which the remainder of the route passes. After entering the city of Timmins, and approximately east of Highway 129, the route meets Highway 144, which travels south to
Sudbury. Classified as a city, accelerated development has occurred progressively in the western portion of the municipality, with the urban portion located in the centre of the city. Through the urban portion, Highway 101 is not maintained as a provincial highway and is a local road. This discontinuity begins just east of Kamiskotia Road and continues for to Gervais Street North. East of Gervais Street North, Highway 101 travels northeast to
Hoyle, passing beside several large
open pit mines on the outskirts of Timmins. It then turn east and crosses the
Frederick House River on the northern shores of
Night Hawk Lake. The route passes through the only agricultural area along its journey, travelling straight for the remainder of the distance to Highway 11 west of Matheson. After a concurrency with Highway 11, Highway 101 branches north into Matheson, where it crosses the
Black River before curving east. Returning to wilderness, the route zig-zags towards the Ontario–Quebec border near
Rouyn-Noranda, travelling several kilometres south of
Lake Abitibi. The roadway continues eastward as Quebec Route 388 into the
Abitibi-Témiscamingue region. With the exception of an undivided urban four lane section through Timmins, from Government Road in Mountjoy to Gervais Road South in Porcupine, the entire highway is two lanes wide. == History ==