MARC runs 58 Penn Line trains during a normal weekday. A majority of these trains (29 each day) operate along a stretch between Union Station in Washington and in Baltimore. An additional five trains run between Union Station and in
Middle River, Maryland, while eleven trains run along the entire corridor between Union Station and Perryville. A single morning train and a single evening train run between Perryville and Penn Station, and a single early morning train runs from Martin State Airport to Penn Station. Unlike MARC's other two lines, the Penn Line operates all throughout the day and well into the night rather than solely during peak hours. On December 7, 2013, the Penn Line also began offering limited weekend service. Penn Line weekend service consists of 9 round trips on Saturday and 6 round trips on Sunday—primarily between Penn Station and Union Station. Several trains extend service to Martin State Airport, and all trains skip . Bike Cars are reconditioned
Sumitomo/Nippon Sharyo MARC IIA single-level commuter railcars. One side of each car's interior is lined with bicycle racks which are arranged to secure 23 full-sized, non-collapsible bicycles, and the other side provides seating for 40 passengers. The Bike Car program was expanded during 2015 to include all weekend trains. These railcars are available on most weekday rush-hour Penn Line trains and on all weekend trains. As with the former Bike Cars, these services are first-come, first served with no additional charge, and the bicyclist must make sure to be able to access the platform of the station they desire. Amtrak's
Acela,
Northeast Regional, and other long-distance trains share tracks along the whole of the Penn Line. Washington Union and Baltimore Penn are the second and eighth busiest Amtrak stations in the country, respectively. Amtrak connections are also available at , , and . MARC passengers with monthly and weekly tickets can ride select Amtrak
Northeast Regional trains during the week only, as part of their cross-honoring agreement. Longer-term plans include construction of new track and extending the line past Washington Union Station to
L'Enfant Plaza station in Washington and from there into
northern Virginia. The
Purple Line light rail system (slated to open in 2027) will connect to all three MARC lines; the transfer to the Penn Line will be at New Carrollton. ==Stations==