Phagocytosis

The history of phagocytosis represents the scientific establishment of immunology as the process is the first immune response mechanism discovered and understood as such. The earliest definitive account of cell eating was given by Swiss scientist Albert von Kölliker in 1849. In his report in Zeitschrift für Wissenschaftliche Zoologie, Kölliker described the feeding process of an amoeba-like alga, Actinophyrys sol (a heliozoan) mentioning details of how the protist engulfed and swallowed (the process now called endocytosis) a small organism, that he named infusoria (a generic name for microbes at the time). The first demonstration of phagocytosis as a property of leucocytes, the immune cells, was from the German zoologist Ernst Haeckel. Haeckel discovered that blood cells of sea slug, Tethys, could ingest Indian ink (or indigo) particles. It was the first direct evidence of phagocytosis by immune cells. Phagocytosis was noted by Canadian physician William Osler (1876), and later studied and named by Élie Metchnikoff (1880, 1883). ==In immune system==

of a phagocyte (yellow, right) phagocytosing anthrax bacilli (orange, left) Phagocytosis is one main mechanisms of the innate immune defense. It is one of the first processes responding to infection, and is also one of the initiating branches of an adaptive immune response. Although most cells are capable of phagocytosis, some cell types perform it as part of their main function. These are called 'professional phagocytes.' Phagocytosis is old in evolutionary terms, being present even in invertebrates. Professional phagocytic cells Neutrophils, macrophages, monocytes, dendritic cells, osteoclasts and eosinophils can be classified as professional phagocytes. Monocytes, and the macrophages that mature from them, leave blood circulation to migrate through tissues. There they are resident cells and form a resting barrier. Dendritic cells also reside in tissues and ingest pathogens by phagocytosis. Their role is not killing or clearance of microbes, but rather breaking them down for antigen presentation to the cells of the adaptive immune system. Among these are receptors that recognise the Fc part of bound IgG antibodies, deposited complement or receptors, that recognise other opsonins of cell or plasma origin. Non-opsonic receptors include lectin-type receptors, Dectin receptor, or scavenger receptors. Some phagocytic pathways require a second signal from pattern recognition receptors (PRRs) activated by attachment to pathogen-associated molecular patterns (PAMPS), which leads to NF-κB activation. Degradation can be oxygen-dependent or oxygen-independent. • Oxygen-dependent degradation depends on NADPH and the production of reactive oxygen species. Hydrogen peroxide and myeloperoxidase activate a halogenating system, which leads to the creation of hypochlorite and the destruction of bacteria. • Oxygen-independent degradation depends on the release of granules, containing enzymes such as lysozymes, Bactericidal permeability-increasing protein, Major basic protein and cationic proteins such as defensins. Other antimicrobial peptides are present in these granules, including lactoferrin, which sequesters iron to provide unfavourable growth conditions for bacteria. Other enzymes like hyaluronidase, lipase, collagenase, elastase, ribonuclease, deoxyribonuclease also play an important role in preventing the spread of infection and degradation of essential microbial biomolecules leading to cell death. Some bacteria, for example Treponema pallidum, Escherichia coli and Staphylococcus aureus, are able to avoid phagocytosis by several mechanisms. ==In apoptosis==

Following apoptosis, the dying cells need to be taken up into the surrounding tissues by macrophages in a process called efferocytosis. One of the features of an apoptotic cell is the presentation of a variety of intracellular molecules on the cell surface, such as calreticulin, phosphatidylserine (from the inner layer of the plasma membrane), annexin A1, oxidised LDL and altered glycans. These molecules are recognised by receptors on the cell surface of the macrophage such as the phosphatidylserine receptor or by soluble (free-floating) receptors such as thrombospondin 1, GAS6, and MFGE8, which themselves then bind to other receptors on the macrophage such as CD36 and alpha-v beta-3 integrin. Defects in apoptotic cell clearance is usually associated with impaired phagocytosis of macrophages. Accumulation of apoptotic cell remnants often causes autoimmune disorders; thus pharmacological potentiation of phagocytosis has a medical potential in treatment of certain forms of autoimmune disorders. ==In protists==

Phagocytosis is used by many protists as a means of feeding, thus constituting phagotrophy. • In some, such as amoeba, phagocytosis takes place by surrounding the target object with pseudopods, as in animal phagocytes. In humans, the amoebozoan Entamoeba histolytica can phagocytose red blood cells. • Ciliates also engage in phagocytosis. In ciliates there is a specialized groove or chamber in the cell where phagocytosis takes place, called the cytostome or mouth. As in phagocytic immune cells, the resulting phagosome may be merged with lysosomes (food vacuoles) containing digestive enzymes, forming a phagolysosome. The food particles will then be digested, and the released nutrients are diffused or transported into the cytosol for use in other metabolic processes. Mixotrophy can involve phagotrophic nutrition and phototrophic nutrition. ==See also==