Phosphoribosylglycinamide formyltransferase

In humans, GARTfase is part of trifunctional enzyme which also includes glycinamide ribonucleotide synthase (GARS) and aminoimidazole ribonucleotide synthetase (AIRS). This protein (110kDa) catalyzes steps 2, 3 and 5 of de novo purine biosynthesis. The proximity of these enzyme units and flexibility of the protein serves to increase pathway throughput. GARTfase is located on the C-terminal end of the protein. Human GARTfase has been crystallized by vapor-diffusion sitting drop method and imaged at the Stanford Synchrotron Radiation Laboratory (SSRL) by at least two groups. The cleft consists of the GAR binding site and the folate-binding pocket. The folate-binding pocket is delineated by pteridine-binding cleft, the formyl transfer region and the benzoylglutamate region which bind the pteridine head and a benzoylglutamate tail connected by a formyl bound nitrogen of fTHF. This folate-binding region has been the subject of much research because its inhibition by small molecules has led to the discovery of antineoplastic drugs. The folate-binding loop has been shown to change conformation depending on the pH of solution and as such human GAR transformylase shows highest activity around pH 7.5–8. Lower pH (~4.2) conditions change the conformation of the substrate (GAR) binding loops as well. == Mechanism ==

Mechanism of purN GARTfase Klein et al. first suggested a water-molecule-assisted mechanism. A single water molecule possibly held in place by hydrogen bonding with the carboxylate group of the persistent Asp144 residue transfers protons from the GAR-N to the THF-N. The nucleophilic nitrogen on the terminal amino group of GAR attacks the carbonyl carbon of the formyl group on THF pushing negative charge onto the oxygen. Klein suggests that His108 stabilizes the transition state by hydrogen bonding with the negatively charged oxygen and that the reformation of the carbonyl double bond results in breaking the THF-N - formyl bond. Calculations by Qiao et al. suggest that the water assisted stepwise proton transfer from Gar-N to THF-N is 80-100 kj/mol more favorable than the concerted transfer suggested by Klein. The mechanism shown is suggested by Qiao et al., who admittedly did not consider surrounding residues in their calculations. Much of the early active site mapping on GAR TFase was determined with the bacterial enzyme owing to the quantity available from its overexpression in E. coli. Using a bromoacetyl dideazafolate affinity analog James Inglese and colleagues first identified Asp144 as an active site residue likely involved in the formyl transfer mechanism. Mechanism of purT GARTfase Studies of the purT variant of GAR transformylase in E. coli found that the reaction proceeds through a formyl phosphate intermediate. While the in vitro reaction can proceed without THF, overall the in vivo reaction is the same. == Involvement in de novo purine biosynthesis ==

GART catalyzes the third step in de novo purine biosynthesis, the formation of N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide (fGAR) by formyl addition to N1-(5-phospho-D-ribosyl)glycinamide (GAR). but in humans it is part of a trifunctional protein of 110 kDa which includes AIRS and GARS functionalities. This protein catalyzes three different steps of the de novo purine pathway. == Disease relevance ==

Cancer target Due to their increased growth rate and metabolic requirements, cancer cells rely on de novo nucleotide biosynthesis to achieve levels of AMP and GMP necessary. Being able to block any of the steps of the de novo purine pathway would present significant reduction in tumor growth. Studies have been done both on the substrate binding and folate binding site to find inhibitors. Down syndrome GARTfase is suspected to be connected with Down syndrome. The gene encoding the trifunctional protein human GARS-AIRS-GART is located on chromosome 21q22.1, in the Down syndrome critical region. The protein is overexpressed in the cerebellum during the postnatal development of individuals with Down syndrome. Typically, this protein is undetectable in cerebellum shortly after birth, but found in high levels in prenatal development. == See also ==