Mechanism of action In general, pumiliotoxins are known as positive modulators of
voltage-gated sodium channels (VGSCs,
membrane proteins). Pumiliotoxin 251D is not such a poison. However, it does block the influx of Na+ ions in mammalian VGSCs. Pumiliotoxin 251D is able to shift the V1/2. This is the potential at which the sodium open probability is half maximal. Both the steady-state activation and inactivation curves of each mammalian VGSCs are shifted to a more negative potential. PTX 251D shifts the V1/2 of insect VGSCs even further than the mammalian VSGCs. This explains why it is especially toxic to insects, like mosquitoes. Furthermore, the presence of PTX 251D results in a six time higher permeability of the VGSCs for K+ ions. This severely disturbs the delicate sodium-potassium equilibrium in the nerve system. The effect of pumiliotoxin 251D on the
voltage-gated potassium channels (VGPCs) currents is quite small. The toxic has an effect on the deactivation kinetics of the potassium channel. It inhibits its inactivation. This effect is still under investigation. PTX 251D also completely inhibits the activity of Ca2+-stimulated
ATPase. This results in a decreased reuptake of Ca2+ and thus a high concentration of free Ca2+ in the organism. This may be related to the
potentiation and
prolongation of
muscle twitch caused by the inhibition. The mechanism of
biotransformation of PTX 251D is still unknown.
Effects Pumiliotoxin is a toxin found in poison dart frogs (genus Dendrobates and Phyllobates). It affects the calcium channels, interfering with muscle contraction in the heart and skeletal muscle. PTX 251D has several effects. It rapidly induces convulsions and death to mice and insects (
LD50 being, respectively, 10 mg/kg and 150 ng/larvae). These convulsions are the result of the uncontrollable distortion of the sodium-potassium equilibrium in the
neurons. This is caused by the inhibition of the VGSCs. It also acts as cardiac depressor, causing cardiac arrest. This can be explained by its negative effect on the cardiac VGSC hNav1.5/β1. Although nothing is known of how well PTX 251D penetrates into the brain where convulsions are originated, the observation of convulsions can be explained through inhibition of VGPCs.
Treatment Symptomatic treatment of PTX 251D poisoning include reducing the convulsions using
carbamazepine. This drug targets the affected VGSCs.
Phenobarbital also shows positive effects by interacting with the affected
Ca2+ channels. Ineffective drugs include
diazepam and
dizocilpine. == References ==