On the night of the fire, the club recorded 577 paid admissions and 150 passes, and the orchestra had 14 members and 5 attendants, bringing a likely final total number of people at the club to 746.
Walter Barnes and His Royal Creolians was performing when the fire started near the main entrance door around 11:00 pm and, fed by
Spanish moss that had been draped over interior's rafters as a decoration, quickly engulfed the structure. To ensure there were no bugs in the decorative moss, it had been sprayed with
FLIT, a petroleum-based insecticide. Under the dry conditions, flammable
methane gas was generated from the moss. The Natchez Fire Department did not yet exist, but two volunteer companies provided one full-time firefighter. One of the men lived and worked from the Phoenix Fire Station, about four blocks from the Rhythm Night Club. The fire station received its first telephone call about the club fire at around 11:15 p.m. The first fire engine arrived within minutes, and the first hose was targeted at the front entrance and upper ceiling of the lobby and the second was sent into a small open window at the rear of the club. The fire was put out after around ten to thirty minutes. 21 of the 24 existing windows had been boarded Blinding smoke made movement difficult. Survivors remembered the burning moss falling from the ceiling and forming a barrier between the dance floor and the exit, with the moss igniting clothing and hair of victims and survivors. The front door was the only exit, and the doors swung inward. Due to the walls being made of metal, and little ventilation for the heat or the smoke, the walls held the heat in the club like an oven. When water from the fire hoses hit the metal siding, it created steam that scalded many victims. Doctors later found that most of the dead had been suffocated by smoke inhalation or crushed as the crowd was pushed to the rear of the building. Most of the bodies were found piled near the rear of the building by the bandstand, the stacked bodies reaching shoulder height. The bandmates who died were John Reed Jr., James Coles, Clarence Porter, Henry Walker, Paul Scott, Calvin Roberts, Jesse Washington, and his vocalist Juanita Avery. Local victims were buried at the Watkins Street Cemetery. In the aftermath of the fire, citizens of Natchez raised more than $5,000 to help the local
Red Cross. ==Aftermath==