• Vicars Apostolic of Florencia • Antonio Torasso (10 January 1952 – 22 October 1960) •
Angelo Cuniberti (18 April 1961 – 15 November 1978) •
José Luis Serna Alzate (15 November 1978 – 9 December 1985) • Bishops of Florencia • José Luis Serna Alzate (9 December 1985 – 8 July 1989), appointed Bishop of Líbano-Honda • Fabián Marulanda López (22 December 1989 – 19 July 2002) • Jorge Alberto Ossa Soto (21 January 2003 – 15 July 2011), appointed bishop of the
Roman Catholic Diocese of Santa Rosa de Osos by
Pope Benedict XVI • Omar de Jesús Mejía Giraldo (27 April 2013 – 13 July 2019); elevated to metropolitan archbishop • Archbishops of Florencia • Omar de Jesús Mejía Giraldo (since 13 July 2019) ==Suffragan dioceses==