DeStefano's first credit in the comics industry was as the creator of the character Captain Electron in
Adventure Comics #479 (March 1981), and then the characters Zeep and Thumbelina in the "
Dial H for Hero" story in
Adventure Comics #483 (July 1981). His first professionally published comics artwork was a one-page parody of the "
I…Vampire" feature in
House of Mystery #306 (July 1982). DeStefano wrote and drew "The Mini (Mis)Adventures of Nick O. Tyme" in
New Talent Showcase #6–12 (June–December 1984). He then teamed with writer
Bob Rozakis to create
'Mazing Man, a character introduced in an eponymous series beginning in January 1986. After the cancellation of that series, the Rozakis-DeStefano team created
Hero Hotline in
Action Comics Weekly #637 (January 1989). After a four-issue run as a back-up feature, Hero Hotline received its own six-issue
limited series. In the 1990s, DeStefano was a frequent contributor to
The Big Book Of series published by
Paradox Press. His other comics work includes credits at
Dark Horse Comics,
Disney Comics,
Fantagraphics Books, and
Marvel Comics. As an animator, DeStefano has worked on such series as
The Ren & Stimpy Show and
Futurama. He inked the
Cage limited series drawn by
Genndy Tartakovsky in 2016–2017. In 2020, DeStefano won the Outstanding Individual Achievement in Animation Emmy for Character Design on the Genndy Tartakovsky series
Primal. In 2024, he contributed art to the
Alex Hirsch book
The Book of Bill. ==Filmography==