Telomeric repeat–containing RNA

Transcription From DNA TERRA is an evolutionarily conserved long-non-coding RNA found in many nucleus-containing eukaryotic cells such as chicken (Gallus gallus domesticus), humans (Homo sapiens), budding yeast (Schizosaccharomyces cerevisiae), fission yeast (Schizosaccharomyces pombe), mice (Mus musculus), zebrafish (Danio rerio), and various plants (Arabidopsis thaliana, et cetera). TERRA is transcribed from DNA as a continuous sequence in most tissues from the subtelomeric and telomeric regions at the end of linear chromosomes. Transcription occurs in the 5' to 3' direction (from the centromere to telomere) and TERRA transcripts are heterogeneous in length, ranging anywhere from 100-bases up to 9-kilobases in length for human cells. The majority of TERRA transcription has been shown to be carried out by RNA Polymerase II (RNAPII), While the exact transcription start site is still a matter of debate, it is widely held that TERRA transcription begins in the subtelomeric region at the end of chromosomes, just before the tandem repeats of the telomere begin. In humans, at least 20 of 23 chromosome ends have been shown to contain CpG islands, which are generally found at or near transcription start sites in the subtelomeric region. Within these CpG island are three repetitive DNA tracts occurring in sequence: a centromere-proximal tract of repeated 61-base-pair (bp) units; second, a more distal tract of 29-bp units; and a third 37-bp unit tract. These highly conserved repetitive elements are known as 61-29-37 Repeats. Additionally, the chromatin organization factors CTCF (CCCTC-Binding Factor), Cohesin, and Rad21 - which are known to aid in the recruitment of RNAPII - have also been shown to bind within 1-2 kilobases of the telomeric tracks just upstream of these CpG islands. While not definitely known, the proximity of these factors immediately upstream of the proposed TERRA transcription start site suggest that they may function as promoter elements for the start of TERRA transcription. Therefore, the vast majority of post-transcriptional variation occurs at the 3' end of the transcript; however this has been shown to vary between species, as well as within individual cells. In humans, approximately 7 to 10% of TERRA transcripts have been shown to be post-transcriptionally polyadenylated, Both of these post-transcriptional modifications contribute to stabilizing TERRA within the cell (half-life ~8 hours) compared to non-modified transcripts (half-life ~3 hours). play an important role in regulating total TERRA expression. Additionally, the Shelterin component TRF1 has been shown to support TERRA transcription. It remains unclear whether TERRA transcripts remain tethered to the telomeres from which they are transcribed, acting in cis, or whether they may move from one telomere to another by some unknown mechanism to regulate their functions (acting in trans). Other Telomeric RNA Transcripts While TERRA currently represents the primary subject of study for RNA transcripts originating from telomeres, a wide variety of alternative transcripts have also been identified as part of the yeast telomeric transcriptome: a transcript complementary to the 5' subtelomeric sequence known as ARRET; a subtelomeric transcript complementary to ARRET known as αARRET; and a C-rich telomeric repeat-containing transcript known as ARIA. == Functions of TERRA ==

Interaction with Telomerase Telomerase is the ribonucleoprotein responsible for adding species-dependent tandem repeat sequences (TTAGGG in humans) to the ends of telomeres. These telomeric repeats function to protect the ends of the chromosome from DNA damage or end-to-end fusion with adjacent chromosomes. Since evidence has also shown that TERRA expression is regulated in a telomere-length-dependent manner, while functioning as a positive regulator of telomerase activity at short telomeres however evidence of this relationship in vivo remains elusive. TERRA & Telomerase at Short Telomeres In seemingly contradictory fashion, it is believed that TERRA may instead coordinate the recruitment of telomerase and encourage subsequent telomere elongation for cells containing short telomeres. Specifically in short telomeres of yeast, evidence suggests that TERRA molecules help to form clusters with the yeast telomerase RNA TLC1. These clusters, known as T-Recs, have been shown to preferentially localize to short telomeres and may therefore encourage telomerase-based elongation. Furthermore, proteins that preferentially bind the UUAGGG repeats within TERRA molecules, such as the heterogeneous nuclear ribonucleoprotein hnRNP1A, have been shown to prevent the direct TERRA-telomerase inhibition found at short telomeres, thus promoting telomerase-mediated elongation. The true nature of TERRA's interaction with telomerase remains incompletely described. However, it remains clear that the ability of telomerase to add telomeric repeats to the ends of chromosomes is intricately intertwined with its interaction with TERRA. Heterochromatin Regulation In general, long non-coding RNAs are known to mediate epigenetic changes on chromatin by functioning as recruiters for chromatin-modifying enzymes to genomic loci. TERRA is thought to play a similar role, recruiting both heterochromatic proteins and associated chromatin-remodeling complexes to telomeres, helping to establish or maintain heterochromatin formation. One manner in which chromatin maintains its condensed hetererochromatic state at the telomere is through the enzymes DNA methyltransferases 1 and 3b (DNMT1/3b). Experiments where these factors have been depleted result in increased TERRA expression levels, suggesting that methylation status of the subtelomeric region may help to regulate the expression of TERRA. Further post-translational modifications of telomeric histones may also play a pivotal role in TERRA regulation. Decreased density of trimethylated histones H3K9me3 and H4K30me3 have been shown to correlate with increased TERRA levels. RNA pulldown experiments have also shown that TERRA associates with heterochromatin proteins, including HP1α, HP1β, and the origin recognition complex (ORC). These factors are known to play a role in establishment of heterochromatin and subsequent transcriptional silencing. TERRA-mimicking oligonucleotides have also been shown to associate with other chromatin remodeling complexes such as the NuA Histone Acetyltransferase Complex, BAF-Type SWI/SNF Nucleosome Remodeling Complex, and the NoRC Chromatin Remodeling Complex. It is believed that TERRA may work to stabilize the interaction of these factors with chromatin to promote heterochromatin formation. Overall, evidence has shown a complex relationship whereby TERRA can both regulate the heterochromatic state and be regulated by the heterochromatic state. Increased density of repressive histone marks that cause chromatin to become more condensed correlate with observed decreases in TERRA expression. Concurrently, epigenetic changes that result in chromatin becoming more euchromatic or "open" in nature correlates with increased TERRA expression. Listed above are factors shown to contribute to modulating TERRA expression, but this is by no means an exhaustive list. Regulation of Telomere Length TERRA has been shown to exist exclusively within the nucleus of eukaryotic cells where it specifically associates with the ends of chromosomes at the telomere. While TERRA has not been shown to be an essential or permanent component of telomere chromatin, it is believed that TERRA's primary function is to transiently maintain the structural integrity at telomeres, either through direct interaction with telomeric DNA or by the binding of associated telomeric proteins. Direct Telomeric Interactions Data has shown that TERRA interacts with telomeric DNA to form G-Quadruplex structures both in vitro and in vivo. These structures form due to the abundance of guanine found in the TERRA transcript and their ability to associate through Hoogsteen hydrogen bonding. The resulting square planar structure is known as a guanine tetrad (also known as a G-tetrad or G-quartet) and when two or more guanine tetrads stack on top of each other, they form a G-quadruplex. These complex structures have been shown help to modulate telomere length through inhibition telomerase's ability to add tandem TTAGGG repeats, specifically in cells with long telomeres. This inhibitive effect seems to function in a telomere length-dependent manner; TERRA expression has also been shown to be cell cycle regulated via the chromatin-remodeling protein ATRX, with the highest expression of TERRA being present at the G1 phase following mitosis and before progressively declining to its lowest in S phase. In cells with long telomeres and high TERRA expression, this could potentially lead to replicative arrest and the eventual collapse of the replication fork, resulting in double-stranded breaks at the telomere. TERRA has also been indicated to be involved with the stabilization of the telomere-protecting Shelterin complex at the ends of telomeres. Evidence shows that TERRA functions together with hnRNPA1 to displace RPA from single-stranded DNA following replication and promotes the loading of the Shelterin component POT1 onto telomere ends. This helps to stabilize the Shelterin complex so that it can properly cap the ends of chromosomes and protect them from recognition by DNA damage signaling pathways. TERRA and the Alternative Lengthening of Telomeres (ALT) Pathway In cells that lack telomerase expression, it is well-established that most cells instead maintain the ends of their telomeres through the recombination-based Alternative Lengthening of Telomeres (ALT) pathway. It is proposed that TERRA may work to delay the onset of cellular senescence in these cells and instead promote mechanisms of homologous recombination, thereby driving the ALT phenotype as an alternative means of maintaining telomere ends. In support of this model, TERRA expression has also been shown relate to cellular ALT-status. Cells that have been experimentally confirmed to utilize the ALT pathway have markedly higher levels of TERRA expression compared to non-ALT cells. and members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family have also been shown to regulate the displacement of TERRA from the ends of telomeres. The ability of these mechanisms to actively coordinate the displacement of TERRA provides evidence that TERRA is not a stable constituent of the telomeric chromatin, but not necessarily unstable itself. Instead, the regulated control of TERRA displacement suggests a link between TERRA localization at the telomere and overall genome integrity. For example, in short telomeres with low TERRA expression, UPF1 and other SMG factors may work in concordance with DNA Polymerase δ to remove TERRA from the telomere during replication and alleviate fork stalling to allow for progression of replicative machinery. Additionally, replication fork stalling and SMG factor recruitment may also aid in telomerase localization to the telomeres where de novo repeats may be added to promote elongation of short chromosomes. == Proposed Role in Human Disease ==

While there are no diseases directly attributed to TERRA dysfunction, a number of diseases related to inappropriate telomere shortening and cellular aging may have dysfunctional TERRA expression as one of their causative factors. Such examples include cancer development and progression, Dyskeratosis Congenita (DC), Idiopathic Pulmonary Fibrosis (IPF), Aplastic Anemia (AA), Astrocytoma, and Cryptogenic Liver Cirrhosis. Immunodeficiency, Centromeric Region Instability, Facial Anomalies (ICF Syndrome) ICF is a rare autosomal recessive disease arising from deficiencies or mutations in DNA Methyltransferase 3b (DNMT3b), which acts as a key modulator in maintaining heterochromatin at telomeres. As a result, many ICF patients experience spontaneous genomic instability in various tissues and systemic immunodeficiency. Contrary to traditionally low TERRA levels at short telomeres it has recently been shown that ICF patients display increased TERRA transcription Further evidence also shows a decrease in trimethylated histone H3K9 at the telomeres and loss of binding of the Shelerin-component TRF2 at the subtelomeres. As a Target for Small Molecules There are currently no known inhibitors that specifically target TERRA in cells. However, relating to many of the diseases and syndromes listed above, it is believed that targeted transcriptional silencing of TERRA may potentially provide a viable mechanism for treatment of cells with high levels of TERRA expression. Increasing the activity of TERRA-repressive histone deacetylases such as SIRT1 and SIRT6 through the use of allosteric activators may be prime candidates for the active repression of TERRA transcription. Targeting TERRA with antisense RNA or RNAi technology may also be a way to promote proper telomere function and preserve telomere length. However it is important to note that complete abolishment of TERRA transcription must be avoided, as TERRA's association with the shelterin component POT1 plays a key role in recruitment of the complex to the ends of telomeres. As a further consideration, decreased TERRA expression on an organismal level may increase the replicative capacity of all actively dividing cells, thus potentially promoting tumorigenesis. Only a therapy targeted to cells with high levels of TERRA expression should be considered in these cases. Since TERRA is also proposed to have a role in telomerase inhibition, factors increasing TERRA transcription may also be useful in select cases where aberrant telomerase activity is a contributing factor of disease. General HDAC inhibitors or specific sirtuin inhibitors such as Nicotinamide or Cambinol may prove to be viable treatment options in these cases. Trichostatin A (a HDAC1/2 inhibitor) has already been shown to significantly increase TERRA levels in human cancer cells. It is important to note that TERRA upregulation alone has not been shown to be sufficient to induce telomere loss, so these drugs may need to be given in combination with other therapies, such as telomerase inhibitors. == See also ==