Following his residency and fellowship, Inglesby became an
assistant professor in the Department of Medicine at the
Johns Hopkins University School of Medicine. and was a principal designer, author, and controller of the
Operation Dark Winter Later, he was also promoted from senior fellow to deputy-director of the Johns Hopkins Center for Civilian Biodefense Studies. In 2003, he joined the
University of Pittsburgh School of Medicine as an
associate professor. In 2009, Inglesby replaced O'Toole as director and chief executive officer of the center. In 2020, Inglesby was recognized as being amongst the 50 most influential clinical executives by
Modern Healthcare. During the
COVID-19 pandemic, Inglesby provided technical guidance to response efforts at the global, federal, state, and local level. He served as a consultant for
Larry Hogan, the Governor of Maryland and sat on the COVID Collaborative’s National Advisory Council and on the National Commission on COVID-19 and Criminal Justice. ==Personal life==