• A dark gray cell indicates the character did not appear in that installment. • A indicates a character appears in a cameo appearance. • An indicates a performer stood in as their character's singing voice. • A indicates an actor or actress was not credited for their respective role.
Main characters •
Roger Rabbit (voiced by
Charles Fleischer): A crazy rabbit, main toon character in the film, and central character in the franchise. •
Eddie Valiant (played by
Bob Hoskins): A private detective, and main human character in the film. •
Jessica Rabbit (voiced by
Kathleen Turner): Roger's attractive wife, and singer in The Ink and Paint Club. •
Judge Doom (played by
Christopher Lloyd): Judge of Toontown, and main antagonist in the film.
Other toons • '''''' (voiced by Lou Hirsch): Roger's co-star in short films. Although he has the appearance of a baby, he is really a middle-aged man. He acts as an innocent baby when acts with Roger in the cartoons (being voiced by
April Winchell as an infant). • '''''': Five weasels who act as the Toontown police force, working under the orders of Judge Doom, acting as the film's secondary antagonists. They also appear as the main antagonists in the ''
Roger Rabbit's Car Toon Spin attraction. The characters are based on the weasels from the film The Adventures of Ichabod and Mr. Toad''. • '''''' (voiced by
David Lander): Leader of the Toon Patrol, and Judge Doom's right-hand man. He is the only one with constant dialogue in the film, unlike his companions, who only have brief phrases. • '''''' (voiced by Charles Fleischer): A weasel notable for wearing a 1930s-style green suit. • '''''' (voiced by Charles Fleischer): A weasel who has bulging eyes, spiky hair, and wearing a straitjacket. • '''''' (voiced by
Fred Newman): A weasel who stands out for being obese, and wearing children's clothing such as a striped T-shirt, sneakers, and a hat with a propeller. • '''''' (voiced by
June Foray): A weasel who stands out for being blue-gray (unlike his companions who are brown) and constantly smoking cigarettes that cause him to be surrounded by smoke. • ''''
(voiced by Charles Fleischer): An anthropomorphic taxi cab that was imprisoned by the Toon Patrol until freed by Roger and Eddie, whom he helps escape from the weasels. He also appears in the Roger Rabbit's Car Toon Spin attraction as the vehicle used by Roger to rescue Jessica. Benny also has cameo appearances in the House of Mouse'' television series episodes "Max's New Car" and "Mickey vs. Shelby". • '''''' (voiced by
Amy Irving): Baby Herman's "mother" in the cartoons starring Roger. She is seen
only from the waist down. • ''''
(voiced by Morgan Deare in the film and Jeff Bergman in Roger Rabbit's Car Toon Spin''): A big gorilla and the
bouncer of The Ink and Paint Club. • '''''' (voiced by
Pat Buttram,
Jim Cummings, and
Jim Gallant): Are a gang of western living cowboys like bullets who were once owned by Eddie Valiant until his brother was murder by a toon and never use them again until 3 years later when Eddie decide to aid them on his case he test the native American bullet to destroy the bottle while he tries to shoot three at Doom but only to misplace him so Eddie got rid of his gun leaving the last two bullets unknown. • '''''' (voiced by
Nancy Cartwright): Is a living Toon shoe who plays a death role when after the authorities accidentally open up the Clown shoes they got everything back in except one who was standing next to Judge Doom while its possible it tried to warn Eddie that Doom is the killer who murder Marvin acme since it lived in the factory as an eye witness but only to get dip by Doom in front of Eddie much to his horror. The Shoe became a Display for Judge Doom during the Oogie Boogie Bash event since 2023. • '''''': Is a handsome man face like sword who was known to sing by the impression of
Frank Sinatra, When Eddie tried to use the Sword to dual towards Doom but all he did was sing Witchcraft which wasted his time and almost his life. Yet it's possible the sword's main ability other than being forced to fence like a real blade was singing victims to sleep whoever has a weak mind but didn't work on Doom due to having a strong mind. • '''''': Benny's "twin cousin", who appears in the ''
Roger Rabbit's Car Toon Spin'' attraction as the ride vehicle used by the guests. Additionally,
Droopy, character from
MGM cartoons, make cameo appearances in both the film (voiced by
Richard Williams) and the three short films (voiced again by Williams in
Tummy Trouble, and by
Corey Burton in the other two).
Other humans • '''''' (played by
Joanna Cassidy): Eddie's girlfriend. She works as a waitress at a bar, where she helps Eddie hide Roger. Later, after the incident between Roger and Doom at the bar, she mentions that her boss fired her. • '''''' (played by
Stubby Kaye): The wisecracking owner of the Acme Factory and Toontown, who is killed after a safe falls on his head. At the climax of the film, it is discovered that in his will he leaves the Toons as the owners of his property. • '''''' (played by
Alan Tilvern): Owner of Maroon Cartoons and Roger's boss. He hires Eddie to find out about Jessica's infidelities. Later, Eddie discovers that it was all a planned ploy, but when Maroon is about to tell him the whole truth, he is killed by being shot by Judge Doom. • '''''' (played by
Richard LeParmentier): Police lieutenant and friend of Eddie, whom he takes to the Acme Factory to show him the scene of Marvin Acme's murder. At the climax of the film, he meets Eddie again at the Acme Factory, where Eddie reveals to him Doom's acts on the case. • '''''' (played by
Richard Ridings): A patron at the bar where Dolores works, who mocks Eddie's job as a detective on toon cases. Although later, he ends up covering up Roger's presence at the bar when Judge Doom looks for him. • '''''': Eddie's deceased brother, with whom he worked as a private detective, until he was killed by a toon who dropped a piano on his head during a case. • ''''
(played by Joel Silver in the film and Sol Pavlosky in Tummy Trouble''): Director of Roger's films, who ends up enraged due to Roger's clumsiness when it comes to acting. == Video games ==