Toxic Crusaders •
Toxie (voiced by
Rodger Bumpass) – The main protagonist of the series. He was originally named Melvin Junko and was a meek and quirky janitor who worked at a health club, who was often toyed with by Bimbette and her friends. When they played a trick on Melvin, which involved him wearing a tutu, he ran in embarrassment and ended up stumbling into a drum filled with toxic waste. Instead of killing him, however, the toxic waste transformed him into a grotesque green-skinned mutant with athletic attributes. Melvin would change his name to Toxie and become the leader of the Crusaders. His weapon of choice is a superpowered mop named Mop, which gained sentience after being exposed to the toxic chemicals. •
Nozone (voiced by
Paul Eiding) – He was originally a test pilot who, after flying through a hole in the
ozone layer and crashing into a silo of radioactive pepper, gained the appearance of a blue-skinned humanoid with a wheel for a right foot. He has the power to emit powerful sneezes from his enormous nose. •
Major Disaster (voiced by
Ed Gilbert) – He was originally a military soldier who gained the ability to control plants after falling into a radioactive swamp. •
Headbanger – A fusion of two opposing personalities into a two-headed body made up of
Dr. Bender (voiced by
Hal Rayle), the cantankerous
mad scientist for the right half, and
Fender (voiced by
John Mariano), the surfer-like singing
telegram boy, for the left half. The Fender part was responsible for the accident that caused them to fall into the atom-smasher and merge into one body. Bender mutated into a green creature akin to Toxie, but Fender retained his human appearance. Headbanger originally worked with Dr. Killemoff in a plot to put chemicals in the food at a fast food restaurant, but defected to Toxie's side after seeing girls preferred the Toxic Crusaders. •
Junkyard (voiced by
Gregg Berger) – Junkyard is a humanoid dog who was formed from a junkyard dog and a homeless man. When they took shelter in Junkyard's kennel, which was covered in toxic waste, lightning struck it and merged them into one being. He joined the Toxic Crusaders after helping them fight Dr. Killemoff's forces when he disguised Island City as a resort.
Villains •
Czar Zoster (voiced by
Patric Zimmerman) – One of the primary villains of the series and the ruler of Smogula. He is a small bug-like Smogulan and Dr. Killemoff's boss. •
Dr. Killemoff (voiced by
Rodger Bumpass) – One of the primary villains of the series, who comes from the planet Smogula. He wears a breathing apparatus resembling a mask to survive on non-polluted worlds. Two recurring gags involving Killemoff are that he always corrects someone when they refer to him as Killemoff by shouting, "That's DR. Killemoff" and that he never listens to Psycho's predictions despite the fact they could help him succeed. •
Psycho (voiced by
Michael J. Pollard) – A sardonic, obese, bio-mechanical humanoid. The reoccurring gag in each episode he appears in involves his ability to poke holes in the logic of Dr. Killemoff's plans and accurately predicting how they will fail. Despite his superior intelligence, he shows no desire or ambition to supersede Dr. Killemoff, preferring to keep his role as a henchman. •
Bonehead (voiced by
Hal Rayle) – A bullying punk who was among those who laughed at Melvin's tutu incident. He later attempts to fight Toxie, who confronts him and his buddies for trying to take Yvonne's accordion. In self-defense, Toxie throws him into a barrel of acid rain, resulting in his near-skeletal appearance. Bonehead then joins forces with Dr. Killemoff and is put in command of the Radiation Rangers. He is based on Bozo from the first movie. •
Mayor Max Grody (voiced by
Chuck McCann) – The corrupt mayor of Tromaville, who is allied with the Smogulans. He is based on Mayor Peter Belgoody from the first movie. •
Mona (voiced by
Susan Blu) – Mayor Grody's secretary. •
Polluto – A living
oil slick monster created by Dr. Killemoff. Toxie manages to destroy Polluto in "The Maxing of Toxie" by having No-Zone sneeze a pile of cat litter on Polluto, causing it to explode. In "Club Fred," Polluto is recreated and unleashed on the Toxic Crusaders. The Toxic Crusaders throw large antacid pills on Polluto, causing it to dissolve. •
General GarBage – A Smogulan general who appears in "Invasion of the Biddy Snatchers.", where he is called in by Czar Zostas to replace Dr. Killemoff. His plot involves having his infiltrators taking over the elderly citizens of Tromaville by biting them and becoming a four-armed clone of the elderly citizens; among the victims are Mrs. Junko and Mayor Grody's mother. When Dr. Killemoff is surprised that General GarBage's plan is working, he states to Psycho and Bonehead that they will be out of a job, resulting in Dr. Killemoff calling the Toxic Crusaders for help. With help from Toxie's mother and the other elderly citizens, the Toxic Crusaders defeat the clones, with the elderly citizens using their hair spray to regress the clones back to normal form. General GarBage then appears and unleashes the Radiation Rangers on the Toxic Crusaders. At the suggestion of his mop, Toxie uses the hair spray to shrink General GarBage, causing him to retreat to Smogula. •
Radiation Rangers – Hunched-over mutants who wear yellow
hazmat suits and gas masks and serve as Dr. Killemoff's foot soldiers.
Other characters •
Blobbie – Toxie's pet, which resembles a blob of goo. Toxie met Blobbie when he first arrived in the toxic waste dump •
Yvonne (voiced by
Kath Soucie in a Bronx accent) – Toxie's girlfriend. She often plays the accordion and sings soprano in a pitch high enough to break things, but Toxie is too polite to criticize her about it. One time, the show broke the
fourth wall, as Yvonne's singing causes the viewer's TV to break in the form of animated broken glass. Toxie apologizes to the viewer and tells them the Toxic Crusaders will buy them new TV sets. She is based on Toxie's girlfriend Claire from Toxic Avenger part 2 and 3 as played by
Phoebe Legere. •
Mrs. Junko (voiced by
Susan Silo in a Brooklyn accent) – Toxie's mother. •
Lloyd – A friend of the Toxic Crusaders. A recurring gag is that his wife and children have a full beard like him. •
Snailman – A man who was originally a race car driver. After careening off a bridge into a barge full of snails, the engine oil merged him with a snail. He once helped the Toxic Crusaders fight Dr. Killemoff and Czar Zoster. •
Mower Man – A man who was originally a gardener. After taking a nap in a garden shed that contained leaking gardening chemicals, which exploded in the heat, he became a half-man, half gardening equipment hybrid. He describes himself as "A hideously deformed creature of superhuman shears and strength." He works with Dr. Killemoff in a plot involving Weed Monsters, but after being fired for accidentally mowing the Weed Monsters, he is last seen applying for a job in the want ads to help Mayor Grody destroy incriminating evidence. ==Episodes==