Wybutosine

(yeast TRM5, human TRMT5) converts G37 to m1G37. • S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase (TYW1), using pyruvate as a C-3 source, forms the tricyclic core of wybutosine with flavin mononucleotide (FMN) as a cofactor. The result is called 4-demethylwyosine (abbreviation symbol imG-14, standing for "imidazomethylguanosine minus 14", or yW-187, for "wybutosine minus 187"). The "minus" indicates that the molecular mass is this many units lower than the derived compound being named. • tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase (yeast TYW2, human TRMT12) transfers the α-amino-α-carboxypropyl group from Ado-Met, a common substrate involved in methyl group transfers, to the lateral side chain at the C-7 position of imG/yW-187 to form yW-86. • tRNAPhe 7(((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N4)-methyltransferase|tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase (TYW3) acts as a catalyst for N-4 methylation of yW-86 to produce yW-72. • TYW4 methylates the α-carboxy group of yW-72 to give yW-57. • TYW4 performs methoxycarbonylation of the α-amino group of the yW-58 side chain to give wybutosine. • Hydroperoxywybutosine (o2yW) has been isolated form plants and animal liver, differing from OHyW by having a hydroperoxyl sidechain instead. As o2yw can form from OHyW during sample preparation, it is unclear whether o2yW is just an unnatural artifact of experimentation. • Other types of cancer cells as well as normal non-mammalian cells use m1G instead. Eukaryotes likely descended from the fusion of an archaeon host cell and a proto-mitochondrion bacterial symbioant. Most archaeons have homologs of Trm5, Tyw1, and Tyw3, called aTrm5, Taw1, and Taw3 respectively. Some have a homolog of Tyw2 called Taw. Based on the distribution of these enzymes among archaeal taxa, it is likely the ancestral archaeon already had these enzymes. As a result, they also exhibit hypermodification of the G37 position of tRNA(Phe), although the lack of TRM4 prevents them from making wybutosine, so they use a different but similar base. yW-86 and yW-72 are yet to be chemically synthesized. Their presence in tRNA is inferred from mass spectroscopy and their structure from that of the final yW. == Function ==

Structural effect When magnesium ions are present, wyobutosine causes a shift in the position in the anticodon loop. The hydrophobic nature of yW causes a preference of UUC over UUU, as Watson–Crick pairing with U is prevented. Avoidance of frameshifting Wybutosine and other unnatural nucleosides have been proposed to lead to a single outcome of hypermodification. This hypermodification at position 37 of tRNAPhe may allow for base- stacking interactions which play a key role in maintenance of the reading frame. Through its large aromatic groups, stacking interactions with adjacent bases A36 and A38 are enhanced, which help to restrict the flexibility of the anticodon. It has been found that when tRNAPhe lacks wybutosine, increased frameshifting occurs. Generally, modifications at position 37 prevent base pairing with neighboring nucleotides by helping to maintain and open the loop conformation, as well as generating an anticodon loop for decoding. A wyosine-type modification of tRNAPhe is found to be conserved in archaea and eukarya but is not found in bacteria. Studies from the 1960s and 1970s noted that many mutations could lead to problems in translational accuracy. Further study of the mechanisms involved in translational accuracy revealed the importance of modifications on positions 34 and 37 of tRNA. Regardless of species, these sites of tRNA are almost always modified. The fact that wybutosine and its various derivatives are only found at position 37 may be indicative of the nature of the phenylalanine codons, UUU and UUC, and their predilection for ribosome slippage. This has led to the assumption that tRNAPhe modification at position 37 correlates with the amount of polyuridine slippery sequences found in genomes. Alternatives Besides the wyosine derivatives detailed above, these bases are also found in tRNAPhe across different types of life: • Isopentenyladenosine i6A, some eukaryotes (cytoplasmic) It has been suggested that frameshifting may be used in a programmed manner, possibly to increase coding diversity. Downstream effects The human gene SMARCAD1 contains many UUU codons. When human embryonic stem cells has the TYW1 gene knocked out, it can only make m1G, causing lower translational efficiency of SMARCAD1. This leads to disinhibition of HERVK, preventing the cell from properly differentiating into a neuron. ==References==