Mitochondrial DNA analysis indicates a deep divergence between a northern clade (containing
T. teguixin,
T. palustris and
T. quadrilineatus) and a southern clade (containing
T. duseni). The northern and southern clades are morphologically distinct, with the northern clade possessing a single pair of
loreal scales between the eye and the nostril and a smooth texture to the scales on the body and the southern clade possessing two pairs of loreal scales and a bumpy texture to the scales on the body. At least one review of the morphology of the family Teiidae has placed the tegus of the southern clade in the genus
Salvator. Subsequent studies support the paraphyletic status of
Tupinambis, though further research will be necessary to determine if the split will gain wider acceptance among the herpetological community. Comparative analysis of
hemipenial anatomy also provides support for the split between
Tupinambis and
Salvator. Tegus probably originated sometime during the Cenozoic era.
Tupinambis fossils from Argentina date back to the
Late Miocene, and further remains are also known from the Pleistocene of northwestern Argentina. Fossils of the extinct tegu
Paradracaena can be found in earlier Miocene deposits. ==References==