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Verhoeff's stain

Verhoeff's stain, also known as Verhoeff's elastic stain (VEG) or Verhoeff–Van Gieson stain (VVG), is a staining protocol used in histology, developed by American ophthalmic surgeon and pathologist Frederick Herman Verhoeff (1874–1968) in 1908. The formulation is used to demonstrate normal or pathologic elastic fibers.

Reagents
Verhoeff's stain is a combination of the following reagents: • HaematoxylinIron(III) chlorideLugol's iodineVan Gieson's stainAcid fuchsinPicric acidSodium thiosulfate ==Variants==
Variants
A common variant is the Masson trichrome and Verhoeff stain, which combines the Masson's trichrome stain and Verhoeff stain. It is sometimes just referred to as a Masson trichrome. This combination is useful for the examination of blood vessels; the Verhoeff stain highlights elastin (black) and allows one to easily differentiate small arteries (which typically have two elastic laminae) and veins (which have one elastic lamina). ==References==
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