The venom of
Vespa luctuosa has the highest recorded toxicity to mice of any wasp species tested. The of the venom is 1.6 mg/kg. The toxicity (measured against mice) per weight of
Vespa luctuosa venom is higher than that of the larger
Asian giant hornet (
Vespa mandarinia), which has an LD50 of 4.0 mg/kg, but which is responsible for many more human deaths than
Vespa luctuosa due to the considerably larger volume of venom injected per sting. In addition to being the most venomous known wasp,
Vespa luctuosa has one of the highest recorded toxicities of any known insect venom. Only harvester ant venom from the genus
Pogonomyrmex (especially
Pogonomyrmex maricopa), as well as the unrelated ant species
Ectatomma tuberculatum, are known to be more toxic. ==Nesting==