Cystovirus

Pseudomonas virus phi6 was the first virus in this family to be discovered and was initially characterized in 1973 by Anne Vidaver at the University of Nebraska–Lincoln. She found that when she cultured the bacterial strain Pseudomonas phaseolicola HB1OY with halo blight infected bean straw, cytopathic effects were detected in cultured lawns, indicating that there was a lytic microbe or bacteriophage present. In 1999, phi7–14 were identified by the laboratory of Leonard Mindich at the Public Health Research Institute associated with New York University. They did this by culturing various leaves in Lysogeny Broth and then plating the broth on lawns of Pseudomonas syringae pv phaseolicola. They were able to identify viral plaques from this and then subsequently sequence their genomes. == Microbiology ==

Structure Cystovirus particles are enveloped, with icosahedral and spherical geometries, and T=13, T=2 symmetry. The virion diameter is around 85 nm. There are many proposed members of this family. Pseudomonas viruses φ7, φ8, φ9, φ10, φ11, φ12, and φ13 have been identified and named, but other cystovirus-like viruses have also been isolated. However, cystoviruses do not only infect Pseudomonas. But also bacteria of the genera Streptomyces, Microvirgula, Acinetobacter, Lactococcus, Pectobacterium, and possibly other bacterial genera. == Taxonomy ==

The family contains the following genera and species (-virus denotes genus): • Alphacystovirus • Alphacystovirus phi8 • Betacystovirus • Betacystovirus phi12 • Deltacystovirus • Deltacystovirus phi2954 • Epsiloncystovirus • Epsiloncystovirus phiNY • Gammacystovirus • Gammacystovirus phi13 • Gammacystovirus phiYY • Orthocystovirus • Orthocystovirus phi6 • Orthocystovirus phiNN • Zetacystovirus • Zetacystovirus CAP Other unassigned phages: • Streptomyces virus phi0 • Lactococcus virus phi7-4 • Pectobacterium virus MA14 • Acinetobacter virus CAP3 • Acinetobacter virus CAP4 • Acinetobacter virus CAP5 • Acinetobacter virus CAP6 • Acinetobacter virus CAP7 == References ==