The museum's galleries focus on the natural and cultural history of South Dakota. •
Lakota Family Camp: A large diorama depicting the prairie life of a Lakota family in the 20th century. It features a full-size canvas tipi, clothing, and tools, primarily from the
David C. Clark Memorial Collection, which was sourced from the family of Father David Clark, an Episcopalian minister to the Sioux on the Lower Brule Reservation. The backdrop is a mural painted by noted Lakota artist
Robert Penn. •
Pioneer Life: Exhibits in the West Exhibit Hall focus on the homesteading era. Highlights include the original
Mydland Claim Shanty, an 8x10-foot shanty built in 1882 by Norwegian ship-carpenter Gabriel Mydland. Nine family members lived in the shanty and an adjoining dugout for a year on their homestead near Lake Preston. It was donated by his grandson, Gordon Mydland. The exhibit also features a recreated
1910 Schoolroom. •
A Time to Remember: This gallery contains pioneer objects from the 1880–1920 immigration period. Its main attraction is a fully restored '''1912 Moline Dreadnought '35' automobile'''. The car was donated by Elwood Olsen, restored by the Friends of the W. H. Over Museum, and sold for $1,700 in 1912. •
Agriculture Exhibit: Features historic farming tools and research on the context of "East River" agriculture in South Dakota. •
Hero the Elephant: The museum displays the full skeleton of Hero, a circus elephant who was tragically killed in Elkton, South Dakota, in 1916 after being spooked during a parade. •
Lewis & Clark - Spirit Mound Learning Center: Features animals, artifacts, and interactive displays related to the
Lewis and Clark Expedition's time in the region, which was a project completed for the 2004 bicentennial. •
Stanley J. Morrow Photographs: The Hall Gallery displays reproductions of photos by Stanley J. Morrow, who documented the
Dakota Territory (1866–1882). The museum's "Friends of the W. H. Over Museum" organization funded the framing of the priceless collection. •
Other Collections: The museum also holds a collection of historic firearms, an Egyptian mummy acquired by Over in 1950, a large collection of Kodak cameras, and an antique 1888 view camera that was restored to working condition in 2019.
Outdoor Exhibits •
Heritage Garden: The grounds surrounding the museum feature a Heritage Garden with a variety of trees, shrubs, and plants that serve as a living extension of the natural history exhibits inside.
Temporary and Rotating Exhibits In addition to its permanent galleries, the museum features temporary and rotating exhibits. In November 2025, the museum opened its "Celebrating Veterans Exhibit" in Sletwold Hall, curated by Dr. Lynn Muller. The exhibit features displays on conflicts from the Civil War to the Gulf War, including memorabilia from the battleship
South Dakota and the Minuteman missile system. == Operations ==