The water opossum seems to have a history dating as far back as to the
Pliocene Epoch. The first Pliocene occurrences were recorded in 1902, 1906, and 1917 when two fragments of left mandibular ramus with alveoli (characterized as MACN 2464 and MACN 3515), as well as the fragment of the distal end of a humerus (MACN 3675) were collected near the city of Parani, Entre Rios Province, Argentina. The mandibular fossils, MACN 2464 and 3515 were known to be from the water opossum because their teeth were nearly indistinguishable from living populations of the organism in present day. Additionally, water opossum teeth are characteristically different from other didelphids, likely because they are the only semi-aquatic marsupials, making these teeth easy to identify.
Holocene subfossil fragments of
Chironectes have been discovered in
São Paulo, Brazil. Also, there are fossil specimens from the late
Pleistocene–recent cave deposits in
Minas Gerais, Brazil, as well as from the late
Pliocene in
Entre Ríos Province, Argentina. ==Subspecies==