Chamberlayne is a surname. Notable people with the surname include:Edward Chamberlayne (1616–1703), English writer Francis Chamberlayne, English politician George Chamberlayne, British politician John Chamberlayne (c.1668–1723), English writer, translator, and courtier John Hampden Chamberlayne (1838–1882), American politician and publisher from Virginia Tankerville Chamberlayne (1840–1924), English Member of Parliament Thomas Chamberlayne (disambiguation) several men with this name William Chamberlayne (poet) (1619–1679), English poet William Chamberlayne (MP) (1760–1829), English Member of Parliament and landowner The Chamberlayne family, an influential family of Weston, Southampton, United KingdomThe Chamberlayne family, an influential family in Virginia, descended from William Chamberlayne (burgess) of Hereford who settled in New Kent County, Colony of Virginia