On 10 February 2015, Tregulova was appointed the director of the Tretyakov Gallery. unofficially media speculated that Lebedeva refused to support the
Annexation of Crimea by the Russian Federation in public. However, Tregulova continued with the ambitious exhibition program which was started under Lebedeva. She organized a number of highly popular exhibitions, starting from the retrospective of
Valentin Serov in 2016 that generated a long queue of visitors. The Tretyakov Gallery, which is a museum of Russian art, also started exhibiting art of other countries. Furthermore, Tregulova was actively extending the museum collection by pieces of contemporary art. While director, Tregulova significantly expanded the exhibition areas. In particular, the building of the museum located on
Krymsky Val was fully transferred to the Tretyakov Gallery. Tregulova ordered a reconstruction project of the building hiring architect
Rem Koolhaas to carry it out. The project was slated started in 2023. She also opened the museum building in
Samara, the first building of the Tretyakov Gallery outside of Moscow. Construction of two more buildings, in
Kaliningrad and
Vladivostok, started as well. In 2015, the first videoguide in sign language, the first one in Russia, was introduced. ==Controversy==