The 70
First,
Second and
Third Division clubs compete from the first round. Each section is divided equally into a pot of seeded clubs and a pot of unseeded clubs. Clubs' rankings depend upon their finishing position in the
1999–2000 season. Therefore, the 20th place from the Premier League in 2000,
Watford was the top seed, and the club newly promoted to the Third Division,
Kidderminster Harriers, were bottom seeds. The first legs took place on 22 and 23 August and the second legs on 5 and 6 September. ==Second round==