On 11 February, Istiklol announced that
Igor Cherevchenko and his coaching staff had all left the club after their contracts had expired. On 26 February, Istiklol confirmed that both
Romish Jalilov and
Shahrom Sulaymonov had left the club at the end of their contracts, and joined
Khosilot Farkhor. On 15 March 2024, Istiklol announced that
Nikola Lazarevic had been appointed as their new Head Coach on a one-year contract. On 16 March, Istiklol announced the return of
Rustam Soirov on a contract until the end of 2025, and the return of
Oleksiy Larin on a contract until the end of the season. The following day, 17 March, Istiklol announced the signing of Mekhron Madaminov from
Torpedo Miass on a contract until the end of 2025, and the signing of
Slavko Lukić. On 18 March, Istiklol announced the return of
Amirbek Juraboev on a contract until the end of 2025. On 19 March, Istiklol announced the signing of
Murilo Souza on a contract until the end of the season. On 20 March, Istiklol announced that Khaydar Sattorov and Salokhiddin Irgashev had joined
Vakhsh Bokhtar on loan for the season, whilst Shukhratdzhon Shonazarov had joined
Khujand on a similar deal. On the same day, Istiklol announced the signing of
Keita Suzuki on a contract until the end of the season from
Daegu. On 21 March, Istiklol announced the signing of
Blessing Eleke on a contract until the end of the season. On 22 May, Istiklol's matches against
Barkchi on 26 May and
Panjshir on 1 June were postponed due to International call ups. On 16 June,
Rustam Yatimov left the club to join
Russian Premier League club
Rostov for a fee in excess of $250,000. On 2 July, Istiklol announced that their contracts with Kabir Salimshoev and
Alidzhon Karomatullozoda had been terminated by mutual consent. On 8 July, Istiklol announced the return of goalkeeper Nikola Stošić, on a two-year contract. On 9 July, Istiklol announced the departure of
Slavko Lukić from the club after his contract expired. On 12 July, Istiklol announced the departure of
Ivan Novoselec and
Dženis Beganović after their contracts had expired, and the signings of Darko Ilieski and
Pavel Nazarenko on a contracts until the end of the season. On 1 August, Istiklol announced the signing of
Ruslan Khayloyev who'd most recently played for
Tyumen. On 3 August, Istiklol announced the signing of
Matthew Millar who'd most recently played for
Macarthur FC and
Dimitar Mitkov from
Lokomotiv 1929 Sofia both on a contracts until the end of the season. On 4 August, Istiklol announced the signing of
Francesco Margiotta who'd most recently played for
Botoșani, to a one-year contract. On 4 October, Istiklol announced the return of
Igor Cherevchenko as their Head Coach. ==Squad==