2022–2023 Gaines has advocated against the inclusion of
transgender women in the women's division of sports. In September 2022, Gaines supported the campaign of
Republican US Senator
Rand Paul by appearing in a TV ad for him, where she shared her criticism of trans women in women's sports. By January 2023, Gaines had participated in a small protest at the
NCAA Convention, appeared in campaign advertisements for former US Senate candidate for the Republican Party,
Herschel Walker, and spoken at a
Donald Trump rally. In March 2023, Gaines was an invited speaker at a
Texas Senate committee in support of legislation that would categorically prohibit transgender college athletes from competing in sports divisions that align with their gender identity. After the event concluded, protesters arrived. Gaines was escorted by law enforcement officers to shelter in a classroom, where she stayed for three hours while protesters continued to demonstrate outside. On June 21, 2023, Gaines appeared as a witness at a
Senate Judiciary Committee hearing focused on safeguarding civil rights for LGBTQ Americans and according to
C-SPAN, shared her "own personal experience competing with trans swimmer Lia Thomas and having to share the same dressing room." Gaines accompanied Oklahoma Governor
Kevin Stitt as he signed an executive order in August 2023 representing
Independent Women's Voice (IWV) which had developed model legislation the Oklahoma bill was based on. The executive order includes a variety of provisions, including a prohibition on transgender women and girls using bathrooms and locker rooms designated for women, a direction to state agencies to use sex assigned at birth to define male and female, as well as definitions for terms such as "man" and "woman." In November 2023, Gaines confirmed working with
FIDE to prevent transgender women from playing in
women's chess. This prompted criticism from
PinkNews for claiming that trans women had an advantage at chess. That month she also testified to the Ohio Senate Government Oversight Committee about her experiences competing with Thomas and on preventing trans women from competing in female sports in Ohio. The House would go on to pass Bill 68 that would "prevent trans athletes from participating in Ohio women's sports and would block doctors from providing gender-affirming care to trans youth."
2024–present In March 2024, Gaines was one of sixteen female student athletes who launched a legal case against the
National Collegiate Athletic Association (NCAA) over the participation of transgender athletes in women's sports. Gaines spoke at the annual Republican Lincoln Day Dinner in May 2024. After the
attempted assassination of Donald Trump in Pennsylvania, Gaines expressed her support for Trump's candidacy. In July 2024, Gaines spoke at the
Republican National Convention in support of Trump. In August 2024, Gaines announced the launch of the Riley Gaines Center at the
Leadership Institute in Arlington, Virginia. On November 18, 2024, Gaines
tweeted that she had voted for Trump. In February 2025, the
United States Department of Health and Human Services (HHS) launched a webpage on women's health that included videos featuring Gaines discussing her involvement with
Executive Order 14201, which conditions federal funding on compliance with policies restricting participation of transgender girls in girls' sports programs. Several outlets have noted that Gaines's activism often includes publicly targeting and insulting transgender individuals, including high school and middle school athletes, on social media to her 1.6 million followers. Gaines's wider position has been expressed several times as being that sports is a gateway to invalidating the wider womanhood and public acceptance of transgender women, with Gaines quoted as saying "The gender ideology movement is a house of cards, and I believe it's lying on that sports issue (...) This will be the card that makes all of it crumble." ==Personal life==