17th century •
James Johnstone, 1st Earl of Hartfell (1602–1653) •
James Johnstone, 2nd Marquess of Annandale (c.1687–1730) •
Sir James Johnstone, 3rd Baronet (1697–1772), Scottish baronet and politician •
John Johnstone (mayor) (1661–1732), 32nd Mayor of New York City •
William Johnstone, 1st Marquess of Annandale (1664–1721), Scottish nobleman •
Sir William Johnstone, 2nd Baronet (1600s–1727), Scottish baronet and politician
18th century •
Charles Johnstone (c. 1719–1800), Irish novelist •
Chevalier de Johnstone (1719 – c. 1791), army officer •
Edward Huggins Johnstone (1791–1850), Senior Judge of the United States District Court for the Western District of Kentucky •
George Vanden-Bempde, 3rd Marquess of Annandale (1720–1792) •
George Johnstone (Royal Navy officer) (1730–1787), British naval officer and Member of Parliament •
George Johnstone (1764–1813) (1764–1813), British politician •
Sir James Johnstone, 4th Baronet (1726–1794) •
James Johnstone (explorer) (c. 1759–1823) British naval officer, acting lieutenant during George Vancouver's 1791–95 expedition •
John Johnstone (East India Company) (1734–1795), Scottish nabob •
Thomas Johnstone (1772–1839), sailor, smuggler and saboteur •
Sir William Pulteney, 5th Baronet, born William Johnstone (1729–1805), Scottish advocate, landowner and politician
19th century •
Alan Johnstone (1858–1932), British diplomat •
Archie Johnstone (1896–1963), Scottish journalist who defected to the Soviet Union; husband of Nancy Johnstone •
Banner Johnstone (1882–1964), British rower •
Barbara Elizabeth Johnstone (1849–1928), English burlesque performer and opera bouffe soprano •
Edward Grahame Johnstone (1899–1946), British World War I flying ace •
Harcourt Johnstone (1895–1945), British Liberal Party politician •
Henry James Johnstone (1835–1907), British portrait photographer and landscape painter in Australia •
James Johnstone (1801–1888) (1801–1888), Scottish Liberal Party politician •
John Johnstone (footballer, born 1869) (1869–1953), Scottish footballer •
Joseph Johnstone (1860–1931), Scottish Liberal politician •
Justine Johnstone (1895–1982), American actress and pathologist •
Lamar Johnstone (1882–1919), American actor and director •
Ralph Johnstone (1886–1910), American aviator who died in an air crash •
Susan Johnstone (1792–1850), British actress •
William Johnstone (VC) (1823–1857), British soldier, recipient of the Victoria Cross
20th century •
Alex or Alexander Johnstone, multiple persons •
Alison Johnstone (born 1965), Scottish politician •
Arthur H. Johnstone, South African scouting official •
Anne Grahame Johnstone (1928–1998), English illustrator •
Anthony "Tony" Johnstone (born 1956), Zimbabwean professional golfer •
Archibald Johnstone (1924–2014), Canadian businessman and retired Senator •
Billy Johnstone, Australian rugby player •
Bobby Johnstone (1929–2001), Scottish footballer •
Brad Johnstone (born 1950), New Zealand rugby union footballer and coach •
Bruce Johnstone (disambiguation), multiple persons •
Campbell Johnstone (born 1980), All Black, New Zealand Rugby Player •
Chris Johnstone (born 1960) • •
Colin Johnstone, (1921–1991) New Zealand rower •
Davey Johnstone (born 1951), rock guitarist and vocalist •
Derek Johnstone (born 1953), Scottish footballer •
Diana Johnstone (born 1934), American political writer •
Dougie Johnstone (born 1969), Scottish footballer •
Ed Johnstone (born 1954), Canadian ice hockey player •
Edward Huggins Johnstone (1922–2013), American federal judge •
Eve Johnstone, (born 1944) Scottish Head of the Division of Psychiatry at the University of Edinburgh •
Frederick Johnston (disambiguation), multiple persons •
Gavin Wildridge Johnstone (1941–1987), Australian ornithologist •
Graeme Johnstone, Australian state coroner for Victoria •
Gwyneth Johnstone (1915–2010), English landscape painter •
Harry Johnstone, English footballer •
Hugh Johnstone (1931–2014), British Army officer •
James Johnstone (disambiguation), multiple persons •
Janet Johnstone (1928–1979), illustrator •
Jay Johnstone (1946–2020), American baseball player •
John Johnstone (baseball) (born 1968), former Major League Baseball player •
Jude Johnstone, American singer-songwriter •
Keith Johnstone (1933–2023), American drama instructor •
Lance Johnstone (born 1973), American football player •
Lew Johnstone (1916–1983), Australian politician •
Mandy Johnstone (born 1972), Australian politician •
Marty Johnstone (1951–1979), New Zealand drug trafficker •
Nancy Bell-Johnstone (born 1959), American biathlete •
Nancy Johnstone (writer) (1906–1951), English writer and humanitarian, wife of Archie Johnstone •
Nathan Johnstone (born 1990), Australian snowboarder •
Parker Johnstone (born 1961), American race car driver •
Paul Neil Milne Johnstone (1952–2004), British poet •
Peter Johnstone (disambiguation), multiple persons •
Phil Johnstone, songwriter, keyboardist, guitarist, and record producer • •
Sam Johnstone (born 1993), English footballer •
Sandy Johnstone (1916–2000), British Air Marshal •
Travis Johnstone (born 1980), Australian rules footballer •
Tyler Johnstone (born 1992), American football player •
William Johnstone (disambiguation), multiple persons ==Scottish clan==