barley before kilning or roasting The basic ingredients of beer are water; a starch source, such as
malted
barley, able to be fermented (converted into alcohol); a
brewer's yeast to produce the fermentation; and a flavouring, such as
hops, to offset the sweetness of the malt. A mixture of starch sources may be used, with a secondary saccharide, such as maize (corn), rice, or sugar, these often being termed
adjuncts, especially when used as a lower-cost substitute for malted barley. Less widely used starch sources include
millet,
sorghum, and
cassava root in Africa, potato in Brazil, and
agave in Mexico, among others. The most common starch source is ground cereal or "grist" – the proportion of the starch or cereal ingredients in a beer recipe may be called grist, grain bill, or simply
mash ingredients. ;Water Beer is composed mostly of water. Regions have water with different mineral components; as a result, different regions were originally better suited to making certain types of beer, thus giving them a regional character. For example,
Dublin has
hard water well suited to making
stout, such as
Guinness; while
Pilsen has soft water well suited to making
pale lager, such as
Pilsner Urquell. The waters of
Burton in England contain
gypsum, which benefits making
pale ale to such a degree that brewers of pale ales will add gypsum to the local water in a process known as
Burtonisation. ;Starch source The starch source in a beer provides the fermentable material and is a key determinant of the strength and flavour of the beer. The most common starch source used in beer is malted grain. Grain is malted by soaking it in water, allowing it to begin
germination, and then drying the partially germinated grain in a kiln. Malting grain produces enzymes that will allow conversion from starches in the grain into fermentable sugars during the mash process. Different roasting times and temperatures are used to produce different colours of malt from the same grain. Darker malts will produce darker beers. Nearly all beer includes barley malt as the majority of the starch. This is because of its fibrous husk, which is important not only in the sparging stage of brewing (in which water is washed over the
mashed barley grains to form the
wort) but also as a rich source of
amylase, a
digestive enzyme that facilitates conversion of starch into sugars. Other malted and unmalted grains (including wheat, rice, oats, and
rye, and, less frequently, maize (corn) and sorghum) may be used. In recent years, a few brewers have produced
gluten-free beer made with sorghum with no barley malt for people who cannot digest
gluten-containing grains like wheat, barley, and rye. ;Hops grown in a hop field,
Hallertau, Germany Hops are the female flower clusters or seed cones of the hop vine
Humulus lupulus, which are used as a flavouring and preservative agent in nearly all beer made today. Hops had been used for medicinal and food flavouring purposes since Roman times; by the 7th century in
Carolingian monasteries in what is now Germany, beer was being made with hops, though it isn't until the thirteenth century that widespread cultivation of hops for use in beer is recorded. Before the thirteenth century, beer was flavoured with plants such as
yarrow,
wild rosemary, and
bog myrtle, and other ingredients such as
juniper berries,
aniseed and
ginger, which would be combined into a mixture known as
gruit and used as hops are now used; between the thirteenth and the sixteenth century, during which hops took over as the dominant flavouring, beer flavoured with gruit was known as ale, while beer flavoured with hops was known as beer. Some beers today, such as
Fraoch by the Scottish
Heather Ales company and
Cervoise Lancelot by the French Brasserie-Lancelot company, use plants other than hops for flavouring. Hops contain several characteristics that brewers desire in beer: they contribute a bitterness that balances the sweetness of the malt; they provide floral, citrus, and herbal aromas and flavours; they have an
antibiotic effect that favours the activity of
brewer's yeast over less desirable microorganisms; and they aid in "head retention", the length of time that the foam on top of the beer (the
beer head) will last. The preservative in hops comes from the lupulin glands which contain soft resins with alpha and beta acids. Though much studied, the preservative nature of the soft resins is not yet fully understood, though it has been observed that unless stored at a cool temperature, the preservative nature will decrease. Brewing is the sole major commercial use of hops. ;Yeast Yeast is the
microorganism that is responsible for fermentation in beer. Yeast
metabolises the sugars extracted from grains, which produces
alcohol and
carbon dioxide, and thereby turns
wort into beer. In addition to fermenting the beer, yeast influences the character and flavour. The dominant types of yeast used to make beer are
Saccharomyces cerevisiae, known as ale yeast, and
Saccharomyces pastorianus, known as lager yeast;
Brettanomyces ferments
lambics, and
Torulaspora delbrueckii ferments Bavarian
weissbier. Before the role of yeast in fermentation was understood, fermentation involved wild or airborne yeasts, and a few styles such as
lambics still use this method today.
Emil Christian Hansen, a Danish biochemist employed by the
Carlsberg Laboratory, developed pure yeast
cultures which were introduced into the Carlsberg brewery in 1883, and pure yeast strains are now the main fermenting source used worldwide. ;Clarifying agent Some brewers add one or more
clarifying agents to beer, which typically
precipitate (collect as a solid) out of the beer along with protein solids and are found only in trace amounts in the finished product. This process makes the beer appear
bright and clean, rather than the cloudy appearance of ethnic and older styles of beer such as
wheat beers. Examples of clarifying agents include
isinglass, obtained from
swim bladders of fish;
Irish moss, a seaweed; kappa
carrageenan, from the seaweed
kappaphycus;
polyclar (a commercial brand of clarifier); and
gelatin. If a beer is marked "suitable for Vegans", it was generally clarified either with seaweed or with artificial agents, although the "Fast Cask" method invented by
Marston's in 2009 may provide another method. ==Brewing process==