Osmia calaminthae has only been observed at eleven sites, primarily within a long, wide area of sandy
Florida scrub located in southern
Lake Wales Ridge in
Highlands County, Florida, and in
Ocala National Forest in
Marion County, Florida. The majority of observations are from various
undeveloped lots in
Lake Placid. This makes it possibly among the most geographically limited and host-specific bees in eastern North America. While it is possible that the bee ranges further than has been observed, there has never been an attempt to comprehensively survey bees in Florida or document their hosts. The area of observation is also known for
endemism. Florida scrub is a unique form of shrub environment located on ridges and knolls of wind-deposited sand. The bee depends primarily on
Calamintha ashei and
Conradina brevifolia as pollen sources. Individual
C. ashei are known to live as long as a decade and are suspected to live longer. They bloom primarily in mid-March through mid-April. Since as few as 20 to 30 mature plants can present thousands of flowers, the species serves as a reliable annual food source for
O. calaminthae.
C. brevifolia, which is more rare than
C. ashei, blooms earlier than
C. ashei. It was discovered in 2020 that the bee nests in the ground. Nests have been found in both the Lake Wales Ridge and the Ocala National Forest. == Conservation status ==