Genetically encoded calcium indicators (GECIs) are powerful tools useful for
in vivo imaging of cellular, developmental, and physiological processes. GECIs do not need to be acutely loaded into cells; instead the genes encoding for these proteins can be introduced into individual cells or cell lines by various
transfection methods. It is also possible to create transgenic animals expressing the indicator in all cells or selectively in certain cellular subtypes. GECIs are used to study neurons,
T-cells,
cardiomyocytes, and other cell types. Some GECIs report calcium by direct emission of photons (
luminescence), but most rely on fluorescent proteins as reporters, including the green fluorescent protein
GFP and its variants (eGFP, YFP, CFP). Of the fluorescent reporters, calcium indicator systems can be classified into single fluorescent protein (FP) systems, and paired fluorescent protein systems. Camgaroos were one of the first developed variants involving a single protein system. Camgaroos take advantage of
calmodulin (CaM), a calcium binding protein. In these structures, CaM is inserted in the middle of
yellow fluorescent protein (YFP) at Y145. Previous mutagenesis studies revealed that mutations at this position conferred pH stability while maintaining fluorescent properties, making Y145 an insertion point of interest. Additionally, the N and C termini of YFP are linked by a peptide linker (GGTGGS). When CaM binds to Ca2+, the effective pKa is lowered, allowing for
chromophore deprotonation. This results in increased fluorescence upon calcium binding in an intensiometric fashion. Such detection is in contrast with ratiometric systems, in which there is a change in the absorbance/emission spectra as a result of Ca2+ binding. A later developed single-FP system, dubbed
G-CaMP, also invokes circularly permuted GFP. One of the termini is fused with CaM, and the other termini is fused with M13 (the calmodulin binding domain of myosin light kinase) The protein is designed such that the termini are close in space, allowing for Ca2+ binding to cause conformational changes and chromophore modulation, allowing for increased fluorescence. G-CaMP and its refined variants have nanomolar binding affinities. A final single protein variant is the CatchER, which is generally considered to be a lower affinity indicator. Its calcium binding pocket is quite negative; binding of the cation helps to shield the large concentration of negative charge and allows for recovered fluorescence. In contrast to these systems are paired fluorescent protein systems, which include the prototypical
Cameleons. Cameleons consist of two different fluorescent proteins, CaM, M13, and a glycylglycine linker. Such shortcomings were abrogated by Q69K and V68L mutations. Both of these residues were close to the buried anionic chromophore and these mutations probably hinder protonation, conferring greater pH resistance. Of growing importance in calcium detection are near-IR (NIR) GECIs, which may open up avenues for multiplexing different indicator systems and allowing deeper tissue penetration. NIR GECIs rely on biliverdin-binding fluorescent proteins, which are largely derived from bacterial
phytochromes. NIR systems are similar to inverse pericams in that both experience a decrease in fluorescence upon Ca2+ binding. RCaMPs and RGECOs are functional at 700+ nm, but are quite dim. A Cameleon analog involving NIR FRET has been successfully constructed as well. A special class of GECIs are designed to form a permanent fluorescent tag in active neurons. They are based on the photoswitchable protein
Eos which turns from green to red through photocatalyzed (with violet light) backbone cleavage. Combined with the CaM, violet light photoconverts only neurons that have elevated calcium levels. SynTagMA is a synapse-targeted version of CaMPARI2. While fluorescent systems are widely used, bioluminescent Ca2+ reporters may also hold potential because of their ability to abrogate autofluorescence, photobleaching [no excitation wavelength is needed], biological degradation and toxicity, in addition to higher signal-to-noise ratios. Such systems may rely on
aequorin and the luciferin coelenterazine. Ca2+ binding causes a conformational change that facilitates coelenterazine oxidation. The resultant photoproduct emits blue light as it returns to the ground state. Colocalization of aequorin with GFP facilitates BRET/CRET (Bioluminescence or Chemiluminescence Resonance Energy Transfer), Such systems can also leverage the self-assembly of luciferase components. In a system dubbed "nano-lantern," the luciferase RLuc8 is split and placed on different ends of CaM. Calcium binding brings the RLuc8 components in close proximity, reforming luciferase, and allowing it to transfer to an acceptor fluorescent protein. To minimize damage to the visualized cells, two-photon microscopy is often invoked to detect the fluorescence from the reporters. The use of near-IR wavelengths and minimization of axial spread of the point function allows for nanometer resolution and deep penetration into the tissue. The dynamic range is often determined from such measurements. For non-ratiometric indicators (typically single protein indicators), it is the ratio of the fluorescence intensities obtained under Ca2+ saturated and depleted conditions, respectively. However, for ratiometric indicators, the dynamic range is the ratio of the maximum FRET efficiency ratio (calcium saturated) to the minimum FRET efficiency ratio (calcium depleted). Yet another common quantity used to measure signals produced by calcium concentration fluxes is the signal-to-baseline ratio (SBR), which is simply the ratio of the change in fluorescence (F - F0) over the baseline fluorescence. This can be related to the SNR (signal to noise ratio) by multiplying the SBR by the square root of the number of counted photons. == Usage ==