Calculus is composed of both inorganic (mineral) and organic (cellular and extracellular matrix) components.
In supra-gingival calculus The mineral proportion of supragingival calculus ranges from approximately 40–60%, depending on its location in the dentition, and consists primarily of
calcium phosphate crystals organized into four principal mineral phases, listed here in order of increasing ratio of phosphate to calcium: •
hydroxyapatite, •
whitlockite, •
octacalcium phosphate, • and
brushite, The organic component is approximately 85% cellular and 15% extracellular matrix. The cells within calculus are primarily bacterial, but also include at least one species of archaea (
Methanobrevibacter oralis) and several species of yeast (e.g.,
Candida albicans). The organic extracellular matrix in calculus consists primarily of
proteins and
lipids (fatty acids, triglycerides, glycolipids, and phospholipids), Trace amounts of host, dietary, and environmental microdebris are also found within calculus, including salivary proteins, plant DNA, milk proteins, starch granules, textile fibers, and smoke particles.
In sub-gingival calculus Sub-gingival calculus is composed almost entirely of two components: fossilized anaerobic bacteria whose biological composition has been replaced by
calcium phosphate salts, and calcium phosphate salts that have joined the fossilized bacteria in calculus formations. The following minerals are detectable in calculus by
X-ray diffraction: •
brushite () •
octacalcium phosphate () • magnesium-containing
whitlockite () • carbonate-containing
hydroxyapatite (approximately but containing some carbonate). ==Calculus formation==