Antonio died on 20 January 1731. The previous day, he had announced that his wife,
Enrichetta d'Este, was pregnant; after his death, a regency council for the potential heir was formed, consisting of Enrichetta, a bishop, the first secretary of state and two gentlemen of the court. It was decided that, should the child be female, the
duchy of Parma would pass to
Charles of Spain (then aged 12), eldest of the three sons of Dorothea Sophie's daughter Queen Elisabeth of Spain. Enrichetta was thus invested as regent of Parma, supported by Imperial troops. Enrichetta's pregnancy was questioned by the queen of Spain though her mother, Dorothea Sophie, who wished to defend the right of Charles, as well as the pope, who wished to annex the duchy to the Papal State. However, Enrichetta was supported by the Emperor, who opposed Spanish influence in Parma. Of the request of Spain, Enrichetta was examined in May 1731 by doctors confirming her pregnancy. The news was reported around Parma and then around the European courts. Her regency could thus continue, with support by the emperor. On 22 July however, the
Second Treaty of Vienna officially recognised the right of Charles as the duke of Parma and Piacenza, pursuant to the
Treaty of London (1718). When Spain demanded that the delivery of Enrichetta should be a public affair, the emperor retracted his support to Enrichetta and discontinued the original plan to arranged a simulated birth. Queen Elisabeth in Spain convinced her mother to have Enrichetta examined again on 13 September 1731; it was then reported that there was in fact no child, and the
House of Farnese was extinct. Charles of Spain was thus recognised as duke, deposing the regency of Enrichetta d'Este. Since Charles was still a minor, his maternal grandmother Dorothea Sophie, was named regent. Dorothea ruled as regent until 1735, when the duchy was ceded to Austria after the
War of the Polish Succession. She died in
Parma in 1748 and was buried at the
Sanctuary of Santa Maria della Steccata. ==Issue==