Desulfobulbus propionicus

Desulfobulbus propionicus was discovered in 1982 by Friedrich Widdel and Norbert Pfenning. Desulfobulbus propionicus was isolated from samples taken from anaerobic mud in a village ditch, pond, and marine mud flat in Germany. All three strains were isolated using the agar shake dilution method on a basal medium with added sulfate, mineral salts, iron, trace elements, bicarbonate, sulfide, and seven vitamins. == Etymology ==

The genus Desulfobulbus can be derived from the Latin words -de meaning from, -sulfo meaning sulfur, and -bulbus meaning onion shaped literally meaning onion-shaped sulfate reducer. The species name propionicus is derived from the organisms electron donor propionate. == Taxonomic and phylogenetic description ==

Desulfobulbus propionicus possesses three strains: 1pr3T, 2pr4, and 3pr10. Similarly, all three strains are Gram-negative, sulfur-reducers with the ability to grow exclusively on lactate or pyruvate without any external electron or carbon sources. What separates 1pr3T from its sister strains is its ability to reduce sulfite and thiosulfate to hydrogen sulfide (H2S); reduce nitrate to ammonia; lastly, its presence of cytochrome types b- and c-. Furthermore, strain 1pr3T differentiated from the others in shape (1pr3T possesses pointed ends compared to ovoid or ellipsoidal shaped ends), motility (1pr3T lacks motility, whereas the others possess flagella), and the presence of fimbriae (2pr4 and 3pr10 strains do not). In terms of the genus Desulfobulbus, the closest relatives of D. propionicus are D. elongatus with an identity of 96.9%, followed by D. rhabdoformis, and then D. mediterraneus and D. japonicas with equal relation respective to the phylogenetic tree constructed using 16S rRNA sequences. == Characterization ==

Morphology Desulfobulbus propionicus is a Gram-negative, ellipsoidal to lemon-shaped bacteria, with an average length of 1.0 to 1.3μm and a width of 1.8 to 2.0μm. Strain 1pr3T was found to encompass a genome size of 3,851,869 bp, with a G-C content of 58.93%. Pagani et al. predicted 3,408 genes in the genome of 1pr3T, with 3,351 genes that encode proteins. The genome contains 57 RNA genes and two rRNA operons. Furthermore, there is 68 pseudo genes which makes up 2.0% of the total genome size. Ecology Desulfobulbus propionicus inhabits anaerobic freshwaters and marine sediments. Among the three strains, they differ in: temperature ranges, optimal temperature, pH range, optimal pH, and NaCl concentration requirements (1pr3T and 2pr4 show slowed growth above a NaCl concentration of 15 g/L, and 3pr10 shows no growth below 15 g/L). == Application ==

Desulfobulbus propionicus can serve as a biocatalyst in microbial electrosynthesis. Microbial electrosynthesis is the usage of electrons by microorganism to reduce carbon dioxide to organic molecules. Desulfobulbus propionicus, when present at the anode, oxidizes elemental sulfur to sulfate, which creates free electrons in the process. The free electrons flow to the organism located at the cathode. The microbe present at the cathode utilizes the electron energy transferred from Desulfobulbus propionicus to create organic matter (e.g. acetate) by reducing carbon dioxide. The use of microbial electrosynthesis has potential to aid in the production and waste maintenance of industrial chemicals and energy production. == References ==