Sir Francis and Lady Knollys produced sixteen children: He was married first to
Dorothy Bray, who was 20 years his senior; and secondly to Elizabeth Howard, daughter of
Thomas Howard, 1st Earl of Suffolk and his second wife
Catherine Knyvett. • Edward Knollys (1546–1580). He was a member of Parliament. • Sir Robert Knollys (1547–1626). Member of Parliament representing
Reading, Berkshire (1572–1589),
Brecknockshire (1589–1604), Abingdon, Oxfordshire (1604, 1624–1625) and finally
Berkshire (1626). He married Catherine Vaughan, daughter of Sir Rowland Vaughan, of
Porthamel. • Richard Knollys (1548 – 21 August 1596). Member of Parliament representing first
Wallingford (1584) and then
Northampton (1588). Married Joan Heigham, daughter of John Heigham, of
Gifford's Hall,
Wickhambrook, Suffolk. •
Elizabeth Knollys (15 June 1549 – c. 1605). Lady Elizabeth married Sir
Thomas Leighton of
Feckenham,
Worcestershire, son of John Leighton of Watlesburgh and Joyce Sutton, in 1578. Her husband served as Governor of
Jersey and
Guernsey. • Maud Knollys (1548 – ?). Died young. • Sir Thomas Knollys (died 1596). Known for service in the
Eighty Years' War (1568–1648). Governor of
Ostend in 1586. Married Ottilia de Merode, daughter of Jean IX de Merode, Sire de Petershem and Margareta van Pallant. •
Sir Francis Knollys "the Younger" (c. 1552 – 1648). Member of Parliament representing first
Oxford (1572–1588) and then
Berkshire (1597, 1625). Married Lettice Barrett, daughter of John Barrett, of
Hanham. Father-in-law of
John Hampden. •
Anne Knollys (19 July 1555 – 30 August 1608). Married
Thomas West, 2nd Baron De La Warr. Mother to
Thomas West, 3rd Baron De La Warr, after whom the state of
Delaware is named. • Catherine Knollys (21 October 1559 – 20 December 1620). Married first Gerald FitzGerald, Baron Offaly (son of
Gerald FitzGerald, 11th Earl of Kildare and
Mabel Browne) and secondly Sir Phillip Butler, of Watton Woodhall. She was the mother of
Lettice Digby, 1st Baroness Offaly. • Cecily Knollys (c. 1560 – ?). No known descendants. • Margaret Knollys. No known descendants. • Dudley Knollys (9 May 1562 – June 1562) ==In literature==