After graduating from high school in
Lynn, Massachusetts, Butler was educated at the
Eastern State Normal School, a teaching college located in Maine. The 1906 yearbook at
South High School, where she had been a teacher, warned that students should avoid taking botany with Eloise Butler if they did not enjoy "10 mile walks through bog and swamp in quest of unobtainable flora", according to an article in the
Minneapolis Star Tribune. In 1911, she retired from her 37-year teaching career to become the first curator of the Minneapolis Wildflower Garden that she had helped to establish. Despite the length of her teaching career, she wrote that "in my next incarnation, I shall not be a teacher". == Botany and gardening ==